FFECT OF RNA RELATED COMPOUNDS ON FRUCTIFICATION OF TRICHOLOMA MATSUTAKE

Abstract：Ribonucleic acid and 5'-nucleotide were tested for fructification of Tricholoma matsutake in the field. Water solutions of RNAs were directly added to the mycelial colony of pine forest floor prior to fructification stage. Content of glucosamine, ergosterol and phospholipid of mycorrhizal fine roots were measured for estimation of mycelial status during growth and fructification stages. Phospholipid content was rapidly increased after RNAs addition. RNA solutions affected to make higher level of the mycelial components than control. As the final results, yield and number of T. matsutake fruit body were dramatically increased by the addition of RNAs during fructification period. This procedure was repeatedly done on the same plots three times in every fructification season, and resulted to all positive effect for RNAs supplement.

Keywords：mushroom, mycorrhiza, RNA stimulation additive

RNA復合物對松茸子實體的作用

大賀祥治　 姚方杰　 趙南爽　 北本豐　 李玉　

摘　要：施用核糖核酸和5'-核苷酸試驗,其對生長于林間的松茸子實體的活化作用.在形成子實體之前將RNA關聯物的水溶液直接灑入帶有菌絲群落的松樹林地表層.為了估計處于生長和形成子實體時期菌絲的狀況對高菌根含量根中核苷、麥角固醇和磷脂的含量進行分析.與對照相比,RNA關聯物的水溶液對提高核苷、麥角固醇和磷脂含量有作用,施用RNA關聯物后9天磷脂含量迅速增加,RNA關聯物有助于形成密集的菌絲群落.試驗最后結果表明在形成子實體的兩周時間內關聯物使松茸子實體的產量和數量顯著增加.

關鍵詞：蘑菇,菌根,RNA刺激物

CLC Number：Q939.96 　Document ID：A

Article ID：1672-6472(2004)04-0555-0562

Foundation Item：Grant-in-Aid for Scientific Research of Japan Society for the Promotion of Science (12660153).

Author Resume：姚方杰,Corresponding author: yaofj@yahoo.com.cn

Author Unit：大賀祥治（九州大學大學院農學研究院森林資源科學部門,福岡,日本,811-2415）　

　　　　　姚方杰（吉林農業大學園藝學院,長春,130118）　

　　　　　趙南爽（忠北大學林產工學科,清州,361-763,韓國）　

　　　　　北本豐（鳥取大學農學部生物資源科學,鳥取,680-0945,日本）　

　　　　　李玉（吉林農業大學菌物研究所,長春,130118,中國）　

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Manuscript Received：2004年5月31日

Manuscript Revised：2004年7月26日

Published：2004年11月22日