【作者】 張丹鳳; 鄭仕棟; 陳國平; 鄭麗珠; 潘裕添;
【Author】 ZHANG Danfeng1,ZHENG Shidong1,CHEN Guoping2,ZHENG Lizhu1,PAN Yutian1 1Department of Biological Sciences and Biotechnology of Zhangzhou Normal University,Zhangzhou,Fujian 363000,China;2Crop Farming Management Station of Zhangzhou AgricultureBureau,Zhangzhou,Fujian 363000,China
【機構】 漳州師范學院生物科學與技術系; 漳州市農業局種植業管理站;
【摘要】 通過正交試驗確定了白背毛木耳(Auricularia polytricha)43-26液體發酵培養基的最優配方(g/L):土豆20、玉米粉20、蛋白胨10和磷酸二氫鉀5;利用超聲波輔助水提醇沉法提取胞內粗多糖,多糖含量為75.5%;多糖粗提物經DEAE-52纖維素離子交換層析和Sephadex G-200層析分離純化,分別得到4個相對均一的純多糖組分AP1、AP2、AP3和AP4。
【Abstract】 A growth medium for maximizing polysaccharide production by submerged cultures of Auricularia polytricha 43-26,consisting of(g/L) 20.0 potato,20.0 corn powder,10.0 peptone and 5.0 KH2PO4,was derived by orthogonal testing.A crude fraction containing 75.5% polysaccharide was obtained by aqueous extraction of ultrasound-treated fungal mycelium and precipitation with ethanol.Crude polysaccharide was separated into four partially purified fractions,AP1,AP2,AP3 and AP4,by DEAE-cellulose 52 and Sephade...
【關鍵詞】 白背毛木耳43-26; 胞內多糖; 提取; 純化;
【Key words】 Auricularia polytricha 43-26; intracellular polysaccharide; extraction; purification;
【基金】 福建省教育廳科技項目(編號:JA08159)的部分研究內容
【分類號】S646.6