【作者】劉秀明; 鄭素月; 圖力古爾; 黃晨陽; 張金霞

【Author】LIU Xiuming1; 2; ZHENG Suyue3; BAU Tolgor1; HUANG Chenyang2; ZHANG Jinxia3 1Institute of Mycology; Jilin Agricultural University; Changchun; Jilin 130118; China; 2Institute of Agricultural Resources and Regional Planning; Chinese Academy of Agricultural Sciences; Beijing 100081; China; 3Department of Agriculture; Hebei University of Engineering; Handan; Hebei 056038; China

【作者機構】吉林農業大學菌物研究所; 中國農業科學院農業資源與農業區劃研究所; 河北工程大學農學系

【摘要】 以白靈側耳(Pleurotus nebrodensis)菌株中農一號(CAAS 1)為試驗材料,研究了溫度脅迫(4℃和35℃)對菌絲體內超氧化物歧化酶(superoxide dismutase,SOD)、過氧化物酶(peroxidase,POD)和過氧化氫酶(catalase,CAT)活性的影響。結果表明,溫度脅迫導致3種保護酶活性均發生顯著的變化。SOD活性隨溫度脅迫時間的延長先急劇升高后下降,在脅迫12 h時均達到最大值(低/高溫脅迫分別為46.23 U/g和50.33 U/g);POD活性呈現先升高后緩慢下降的趨勢,在脅迫處理24 h時達到最高值(低/高溫脅迫分別為6.86 U/g和7.22 U/g);CAT活性在脅迫處理12 h內迅速上升,之后平穩上升。

【Abstract】 Superoxide dismutase(SOD),catalase(CAT) and peroxidase(POD) activities were determined over 60 h time courses in extracts of Pleurotus nebrodensis(strain CAAS 1) mycelia incubated at 4 ℃,25 ℃(controls) or 35 ℃ following growth at 25 ℃ for 14 d.SOD activities in extracts of mycelia incubated at 4 ℃ and 35 ℃ increased markedly during the first 12 h following temperature shift and declined thereafter,whereas very little fluctuation in enzyme activity was observed in extracts of mycelium continually maintained at 25 ℃.POD activity increased in extracts of mycelia incubated at all three temperatures and reached peak values after 24 h before declining throughout the next 24 h in mycelium incubated at 35 ℃ and throughout the next 36 h in mycelia incubated at both 4 ℃ and 25 ℃.CAT activity in extracts of mycelia incubated at 4 ℃and 35 ℃ also increased markedly during the first 12 h following temperature shift and continued to slowly increase in the latter case.However,enzyme activity in extrac...更多ts of mycelium continually maintained at 25 ℃ remained at much lower,relatively constant,levels throughout.CAT and POD activities in mycelia incubated at 4 ℃ and 35 ℃ were significantly(P <0.01) higher than values recorded in mycelium maintained at the original growth temperature(25 ℃) throughout the 60 h monitoring period.SOD activities were significantly(P <0.01) higher for 48 h and 36 h in extracts of mycelia incubated at 35 ℃ and 4 ℃,respectively compared with values recorded in mycelium maintained at the original growth temperature(25 ℃).Our data show that temperature shifts to higher and lower values have a significant effect on the activities of antioxidative enzymes in P.nebrodensis mycelia.  還原

【關鍵詞】 白靈側耳; 溫度脅迫; 超氧化物歧化酶; 過氧化物酶; 過氧化氫酶

【Key words】 Pleurotus nebrodensis; temperature stress; superoxide dismutase; peroxidase; catalase

【基金】國家科技支撐計劃項目(編號:2008BADA1B02)的部分研究內容

【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2010年02期

【DOI】CNKI:SUN:SYJB.0.2010-02-013

【分類號】S646.14