【作者】李洪榮; 陳美元; 廖劍華; 王澤生
【Author】LI Hongrong; CHEN Meiyuan; LIAO Jianhua; WANG ZeshengEdible Fungi Institute of Fujian Academy of Agricultural Sciences; Fuzhou; Fujian 350014; China
【作者機構】福建省農業科學院食用菌研究所
【摘要】 采用同工酶聚丙烯酰胺凝膠電泳(polyacrylamide gelelectrophoresis,PAGE)技術,對雙孢蘑菇(Agaricus bisporus)菌株0214及其分解基質能力退化菌株0214-3、0214-5在4種不同液體培養基中培養的菌絲體的酯酶(esterase,EST)、多酚氧化酶(polyphenoloxidase,PPO)、細胞色素氧化酶(cytochrome oxidase,COD)同工酶進行分析,結果表明:在部分培養基中3個菌株的EST和PPO同工酶表型有明顯差異,且2個退化菌株的同工酶表型變異一致,說明雙孢蘑菇分解基質能力的退化與EST和PPO同工酶活性存在相關性。各菌株在不同培養基中的COD同工酶酶譜表型一致。
【Abstract】 Esterase(EST),polyphenoloxidase(PPO) and cytochrome oxidase(COD) isozyme patterns were determined in Agaricus bisporus,strain 0214,and two derived mutant strains(0214-3 and 0214-5) with diminished ability to degrade the cultivation substrate,following growth on four different liquid media.Although the EST and PPO isozyme patterns of the two mutants were virtually identical under every growth condition,they differed from the EST and PPO isozyme patterns of strain 0214 observed following growth on two and three test media,respectively.All three strains exhibited the same COD isozyme patterns irrespective of the growth conditions.Our data indicated a link between EST and PPO isozyme patterns and the substrate-decomposing ability of A.bisporus.
【關鍵詞】 雙孢蘑菇; 聚丙烯酰胺凝膠電泳; 退化; 同工酶
【Key words】 Agaricus bisporus; cultivation substrate-degrading ability; isozyme; esterase; polyphenoloxidase
【基金】國家自然科學基金項目(編號:30871769);; 福建省科技重大專題(編號:2008SZ0002)的部分研究內容
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2010年02期
【DOI】CNKI:SUN:SYJB.0.2010-02-010
【分類號】S646.11