【作者】顏軍; 李濤; 馬紹賓; 趙之偉
【Author】YAN Jun1; 2; LI Tao1; MA Shaobin3; ZHAO Zhiwei11Laboratory for Conservation and Utilization of Bio-resources; and Key Laboratory for Microbial Resources of theMinistry of Education; Yunnan University; Kunming; Yunnan 650091; 2Kunming Center for Disease Control andProtection; Kunming; Yunnan 650091; 3School of Life Sciences; Yunnan University; Kunming; Yunnan 650091; China
【作者機構】云南大學省部共建生物資源保護與利用國家重點實驗室培育基地微生物多樣性可持續利用教育部重點實驗室; 昆明市疾病預防控制中心; 云南大學生命科學學院
【摘要】 采用組織分離法從市售牛肝菌子實體分離得到兩株性狀穩定的分離株Lq4-2和Be2425,利用ITS序列分析并結合分離子實體的形態特征對兩分離株進行鑒定,并對其基礎生物學特性進行了研究。分離株Lq4-2鑒定為牛肝菌科(Boletaceae)成員,分離株Be2425鑒定為廣義美味牛肝菌(Boletus eduliss.l.);兩分離株能在PDA培養基和MRD合成培養基上良好生長;菌絲生長的適宜溫度為2528℃,適宜pH為56,最佳氮源為酒石酸銨;Lq4-2的最佳碳源為葡萄糖,而Be2425的最佳碳源為果糖和葡萄糖。
【Abstract】 Two bolete strains,Lq4-2 and Be2425,were isolated using standard tissue culture methodology from fruit bodies purchased at two local markets,and assigned to the Boletaceae and Boletus edulis sensu lato,respectively based on internal transcribed spacer(ITS1-5.8S-ITS2) sequence analysis.Both strains exhibited highest mycelial growth rates and growth vigor on potato dextrose agar,although several other media also supported fungal growth.Both strains grew optimally between 2528 ℃,between pH 56,and with ammonium tartrate as the N source.Among several carbon sources tested,glucose supported the highest Lq4-2 growth rates,and either fructose or glucose in the case of Be2425.
【關鍵詞】 牛肝菌; 組織分離株; 轉錄間隔區; 生物學特性
【Key words】 Bolete isolates; internal transcribed spacer(ITS) sequences; biological characteristics
【基金】國家“十一五”科技支撐項目(編號:2008BADA01);; 云南省應用基礎研究項目(編號:2005C0003M)的部分研究內容
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2010年02期
【DOI】CNKI:SUN:SYJB.0.2010-02-006
【分類號】S646.3