【作者】潘慧; 徐章逸; 邊銀丙
【Author】PAN Hui1; XU Zhangyi1; BIAN Yinbing1; 21Institute of Applied Mycology; Huazhong Agricultural University; Wuhan; Hubei 430070; China; 2 Research Center of Edible Mushroom Engineering and Technology of Hubei; Wuhan; Hubei 430070; China
【作者機(jī)構(gòu)】華中農(nóng)業(yè)大學(xué)應(yīng)用真菌研究所; 湖北省食用菌工程技術(shù)研究中心
【摘要】 以杏鮑菇(Pleurotus eryngii)6號(hào)和ACCC50894為親本,分別采用單孢雜交和原生質(zhì)體單核體雜交方式獲得65和37個(gè)雜交子,觀測(cè)雜交子及親本菌絲體生長(zhǎng)情況,選擇長(zhǎng)勢(shì)好及生長(zhǎng)快速的雜交子進(jìn)行栽培出菇試驗(yàn)。采用灰色關(guān)聯(lián)度分析法對(duì)15個(gè)生長(zhǎng)勢(shì)較強(qiáng)的雜交子從7個(gè)性狀方面進(jìn)行綜合評(píng)價(jià),結(jié)果表明,單孢雜交獲得的雜交子D12、D18、D20、D30、D19、D31、D17和D48關(guān)聯(lián)度排名先于兩親本,農(nóng)藝性狀和子實(shí)體性狀表現(xiàn)較好;原生質(zhì)體單核體雜交菌株Y29和Y30關(guān)聯(lián)度排名介于兩親本之間。
【Abstract】 Mating experiments involving either monokaryotic basidiospores or protoplast monokaryons derived from Pleurotus eryngii parental strains No.6 and ACCC50894 were used to generate 65 and 37 hybrid strains,respectively.Fruiting tests were carried out on hybrids selected on the basis of mycelial growth rate and growth vigor,and fruit bodies were successfully obtained from 15 hybrids.Grey correlation analysis based on a comprehensive evaluation of seven selected agronomic traits revealed that hybrids D12,D18,D20,D30,D19,D31,D17 and D48 obtained from monospore crossings were superior to the two parental strains,while hybrids Y29 and Y30 obtained from protoplast monokaryon crossings ranked between the two parental strains.
【關(guān)鍵詞】 杏鮑菇; 單孢雜交; 原生質(zhì)體單核體雜交; 農(nóng)藝性狀; 灰色關(guān)聯(lián)度分析
【Key words】 Pleurotus eryngii; monospore crossbreeding; protoplast monokaryon crossbreeding; agronomic character; grey correlation analysis
【基金】公益性行業(yè)科技(農(nóng)業(yè))專項(xiàng)(編號(hào):NYHYZX07-008);; 武漢市科技產(chǎn)業(yè)化專項(xiàng)(編號(hào):20062001011)的部分研究?jī)?nèi)容
【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2010年02期
【DOI】CNKI:SUN:SYJB.0.2010-02-005
【分類號(hào)】S646.1