羅聯忠; 林樹錢; 謝寶貴; 林志彬
福州綠谷生物藥業技術研究所; 福州綠谷生物藥業技術研究所; 福建農林大學菌物研究中心; 福州綠谷生物藥業技術研究所 福州350002; 福建農林大學菌物研究中心; 福州350002 ; 福州350002 ; 福州350002 ; 福州350002; 北京大學醫學部藥理系; 北京100083
【中文摘要】 CTAB法提取靈芝菌絲體總DNA,再以核糖體DNA轉錄間隔區(ITSⅡ、IGRⅠ-PCR RFLP)結合隨機引物擴增多態性(RAPD)標記分析30個靈芝菌株間的親緣關系。結果表明,ITSⅡI、GRⅠ-PCR RFLP產生了47條帶,其中42條顯示出多態性,通過聚類分析將30個菌株分成七大類,包括紫芝、黑靈芝、樹舌靈芝、薄樹靈芝四大類,以及另外三個無法確定具體歸屬的類群,但沒能將赤芝與松杉靈芝區分開。RAPD從247個隨機引物中篩選出8個隨機引物,共檢測到69個多態性位點并全部顯示出多態性,通過聚類分析將供試的30個靈芝菌株全部區分開。
【英文摘要】 Total DNA of Ganoderma mycelium was extracted by the method of CTAB,and genetic relationships among thirty Ganoderma strains were analyzed based on ITS Ⅱ、IGR Ⅰ-PCR RFLP and RAPD.The results showedthat 47 bands were obtained based on ITSⅡ,IGR Ⅰ-PCR RFLP,and 42 bands of them were polymorphic.Thirty strains were divided into seven species: Ganoderma sinense,Ganoderma atrum,Ganoderma applanatum,Ganaderma capense(Loyd) Teng and other three groups(their species are still unknown) through dendrogram aralysis.Only 8 primers produced stable results among 247 random primers by RAPD.Total 69 loci were obtained by using 8 primers and all of them were polymorphic. Based on these 69 loci,thirty Ganoderma strains were differentiated.
【中文關鍵詞】 靈芝; 核糖體DNA轉錄間隔區; 隨機引物擴增多態性
【英文關鍵詞】 Ganoderma; Internal transcribed spacer(ITS); Rondom amplified polymorphic DNA(RAPD)
【基金】綠谷集團有限公司靈芝專項科研資金全額資助項目“靈芝菌種DNA鑒別”(項目編號:2003SHGV01);; 福建省星火計劃項目“福建食用菌集聚產業科技服務體系建設示范——食用菌種質資源庫建設、分類與認定前期技術研究”(項目編號:2003EAT20021)的部分研究內容
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2005年03期 【DOI】CNKI:SUN:SYJB.0.2005-03-001