Polymorphisms of Genetic Characters in Sporulated Monocaryotic Strains of Auricularia Auricula

Abstract：We studied the relationship between the polymorphisms of the mycelial growth vigor and growth rate with the mating type of 38 sporulated monocaryotic strains, esterase patterns polymorphism of 14 sporulated monocaryotic strains, carboxymethyl cellucose(CMC) enzyme relative activities of 10 sporulated monocaryotic strains of Auricularia auricula. The results showed that polymorphism in the mycelial growth vigor and growth rate existed among the sporulated monocaryotic A. auricula strains tested. There was no serious deviation in the 11 ratios between mating type A and mating type a. Ten marker bands of esterase isozyme with different movement rates and 70 bands were screened in 14 tested strains. Compared with parental bicaryotic strain of Au-8, 5 new bands emerged. The CMC enzyme relative activities of 10 sporulated monocaryotic strains of A. auricula were not regular. It was therefore concluded that there was no significant relationship between the mycelial growth vigor and rate with the mating type, and that significant variation was produced among the sporulated monocaryotic strains of A. auricula.

Keywords：Auricularia auricula; sporulated monocaryotic strains; polymorphism; culture characteristics; esterase isozyme; CMC enzyme relative activity

木耳孢子單核菌株遺傳性狀多態性研究

何培新　 羅信昌　 楊繩桃　 張長鎧　

摘　要：研究了38個木耳孢子單核菌株的菌絲生長勢、菌絲生長速度和其交配型,14個單核菌株的酯酶同工酶以及10個單核菌株的羧甲基纖維素(CMC)酶相對活性,分析了菌絲生長勢和菌絲生長速度與交配型的關系.結果表明,供試的木耳孢子單核菌株之間在研究的各遺傳性狀均出現了一定程度的多態性.在測定的38個孢子單核菌株中,交配型A有21個菌株,交配型a有17個菌株,A∶a=21∶17.經χ2檢驗,兩種交配型未出現1∶1嚴重偏離.木耳孢子單核菌株菌絲生長勢和菌絲生長速度與其交配型沒有明顯相關性.在14個單核菌株中,檢測到10種具有不同遷移率的酯酶同工酶標記譜帶.與親本雙核菌株相比,出現了5條新的譜帶.10個木耳孢子單核菌株的CMC酶相對活性呈不規律分布.

關鍵詞：木耳; 孢子單核菌株; 培養性狀; 酯酶同工酶; CMC酶; 多態性

CLC Number：S646.6;Q933

Foundation Item：Supported by the Open Project of State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University

Author Resume：He Peixin, male,born in 1970,B.S.,associate professor,Ph.D student in College of Life Science Shandong University,Jinan 250100

Author Unit：何培新（華中農業大學農業微生物農業部重點實驗室,武漢,430070;山東大學生命科學學院,濟南,250100）　

　　　　　羅信昌（華中農業大學農業微生物農業部重點實驗室,武漢,430070）　

　　　　　楊繩桃（華中農業大學植物保護系中心實驗室,武漢,430070）　

　　　　　張長鎧（山東大學生命科學學院,濟南,250100）　

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華中農業大學學報

JOURNAL OF HUAZHONG AGRICULTURAL UNIVERSITY

2004 Vol.23 No.1