木耳栽培菌株酯酶同工酶的酶譜多樣性研究

邊銀丙　羅信昌　周啟

摘要：采用聚丙烯酰胺凝膠電泳技術對木耳（Auricularia auricula （Hook） Underw）10個栽培菌株酯酶同工酶的酶譜多樣性進行了研究。10個供試菌株的幼齡菌絲（7d）中僅檢測到25條酶帶，各個菌株分別具有2～3條酶帶，10個菌株僅有2種酶譜類型；老齡菌絲（72d）中共檢測到44條酶帶，各個菌株分別具有3～6條酶帶，10菌株共有8種酶譜類型。研究表明，木耳雙核體菌絲中某些酯酶同工酶基因位點在一定的發育時期才開始表達，老齡菌絲酯酶同工酶酶譜在木耳菌株鑒別和遺傳育種研究中具有更大的應用價值。

關鍵詞：木耳，栽培菌株，酯酶同工酶，菌絲體發育，酶譜多樣性

中圖分類號：Q939.96　文獻標識碼：A

文章編號：1007-3515（2000）01-0087-0090

ESTERASE ISOZYME ZYMOGRAM POLYMORPHISMS OF

CULTIVATED STRAINS IN AURICULARIA AURICULA

BIAN Yin-Bing，LUO Xin-Chang

(Institute of Applied Mycology, Huazhong Agricultural University, Wuhan 430070)

BIAN Yin-Bing，ZHOU Qi

(Life Science and Technology College, Huazhong Agricultural University, Wuhan 430070)

ABSTRACT: Esterase isozyme zymogram polymorphisms of 10 cultivated strains in Auricularia auricula (Hook) Underw were investigated using vertical slab polyacrylamide gel electrophoresis. There were 25 bands of esterase isozyme in the zymograms of young mycelia (7d) of 10 tested strains, each of 10 strains had 2 or 3 bands respectively. The 10 strains were divided only into two groups according to their zymogram phenotypes. The esterase isozyme zymograms of old mycelia (72d) were significantly different from those of young mycelia, 44 bands existed in their zymograms, and each strain had 3～6 bands respectively. The 10 cultivated strains were divided into eight groups according to their zymogram phenotypes. This result revealed that some esterase isozyme genes of dikaryon begin to express in certain developmental stages. The esterase isozyme zymogram of old mycelia is more adaptive than that of young mycelia to be used in the strain identification. genetic distance analysis and genetic breeding of A. auricula.

KEY WORDS: Auricularia auricula, Cultivated strain, Esterase isozyme, Mycelial development, Zymogram polymorphisms.

邊銀丙（華中農業大學應用真菌研究所　武漢　430070）（華中農業大學生命科技學院　武漢　430070）

羅信昌（華中農業大學應用真菌研究所　武漢　430070）

周啟（華中農業大學生命科技學院　武漢　430070）

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