香菇正反雙單雜交后代的遺傳分析

葉明　 潘迎捷　 馬桂榮　 陳詠萱

摘　要：選用香菇的雜交菌株農1與野生株Q進行正反雙單雜交,得到6個雜交后代。結果表明：3個正交菌株與3個反交菌株在酯酶同工酶與DNA水平上具有極高的相似性，而對殺菌劑和溫度的敏感性顯示了明顯的遺傳差異，且農藝性狀遺傳差異也十分顯著。正反雜交菌株在核基因相同情況下的遺傳差異應主要歸于細胞質差異。本研究表明，香菇雙單雜交后代是同質異核體。

關鍵詞：香菇, 正反雙-單雜交, 酯酶同工酶, RAPD, 敏感性, 農藝性狀, 同質異核體

分類號：Q939.96　文獻標識碼：A

文章編號：1007-3515（2001）01-0094-0099

GENETIC ANALYSIS OF THE HYBRIDS OF THE POSITIVE AND NEGATIVE DI-MON MATING IN LENTINULA EDODES.

YE Ming（State Key Lab.of Microbiol.Technol.,Shandong Univ.,Jinan 250100;Department of Biological and Chemical Engineering,Anhui College of Mechanical and Electrical Engineering ,Wuhu 241000）　

PAN Ying-Jie（Laboratory of Genetic and Breeding of Mushroom,Agricultural Ministry of China;Shanghai 201106）　

MA Gui-Rong（State Key Lab.of Microbiol.Technol.,Shandong Univ.,Jinan 250100）　

CHEN Yong-Xuan（Department of Microbiology, Nanjing Agricultural University, Nanjing 210095)）　

Abstract：The positive and negative di-mon mating of a crossed strain Nong1 and a wild strain Q from Lentinula edodes were carried out and six hybrids were obtained. The results show that three strains of the positive cross and three strains of the negative cross exist high similarity on the esterase isoenzyme and DNA molecular level. However, they show distinct genetic differences on sensitivity to prochlorac (germicide)and temperature.The agronomic characters of the hybrids are also evidently diffrenet . Clearly, the genetic differences of hybrids of the positive and negative di-mon mating should be attributed to the differences of cytoplasm. This study indicate that the positive and negative di-mon mating hybrids are homogenous heterokaryons in Lentinula edodes .

Keywords：Lentinula edodes, The positive and negative di-mon mating, Esterase isoenzyme, RAPD, Sensitivity , Agronomic character , Homeonomous heterokaryon.

基金項目：本文由博士點基金項目與安徽省教育廳科研基金資助

作者單位：葉明（山東大學微生物技術國家重點實驗室 濟南 250100;安徽機電學院生物化工系 蕪湖 241000）　

　　　　　潘迎捷（食用菌遺傳育種農業部部級重點實驗室 上海201106）　

　　　　　馬桂榮（山東大學微生物技術國家重點實驗室 濟南 250100）　

　　　　　陳詠萱（南京農業大學微生物系 南京 210095）　

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