MOLECULAR TAXONOMIC RELATIONSHIPS OF AURICULARIA SPECIES INFERRED FROM RAPD MARKERS

Abstract：Molecular taxonomic relationships of the genus Auricularia, together with outgroup of Tremella, were studied using random amplified polymorphic DNA. Eight species of Auricularia and two species of Tremella were examined. The dendrogram derived from RAPD data by UPGMA suggested that the genera Auricularia and Tremella would be monophyletic. For Auricularia, three distinct genetic groups were recognized: the first included A. Peltata and A. Cornea; the second included all strains of A.auricula; the third included five morphological species of A. Delicata, A. Fuscosuccinea, A. Polytricha, A. Maxima and A. Reticulata. This molecular taxonomy suggests that A.cornea and A. Polytricha would be two different species and not synonymous, and the presence or absence of a medulla in cross-section of the basidiocarps would not indicate whether or not taxa are closely related.

Keywords： Jelly fungi, Molecular taxonomy, Heterobasidiomycetes, Auriculariales

利用RAPD標(biāo)記構(gòu)建木耳屬種間關(guān)系的研究

閆培生　 蔣家慧　 王東昌　 羅信昌　 周啟　

摘　要：利用RAPD標(biāo)記以銀耳屬的2個(gè)種為外類群研究了木耳屬8個(gè)種的分子系統(tǒng)發(fā)育關(guān)系.根據(jù)UPGMA 構(gòu)建的樹(shù)狀圖結(jié)果表明木耳屬(Auricularia)和銀耳屬(Tremella)分別為單一的分類單元.木耳屬的8個(gè)種被分成3個(gè)明顯不同的組,第一組包括盾形木耳(A.peltata Lloyd)和角質(zhì)木耳(A.cornea Spreng.),第二組包括所有供試的黑木耳(A.auricula Underw.),第三組包括皺木耳(A.delicata Henn.)、琥珀木耳(A.fuscosuccinea Farl.)、毛木耳(A.polytricha Sacc.)、大木耳(A.maxima Y. R. Guo)和網(wǎng)膜木耳(A.reticulata L. J. Li)五個(gè)形態(tài)種.種間發(fā)育關(guān)系表明角質(zhì)木耳和毛木耳應(yīng)是兩個(gè)不同的種而非同種異名,子實(shí)體橫切面中髓層的有無(wú)并不表明不同種間親緣關(guān)系的遠(yuǎn)近.

關(guān)鍵詞：膠質(zhì)真菌,分子系統(tǒng)學(xué), 異隔擔(dān)子菌,木耳目

分類號(hào)：Q939.96 　文獻(xiàn)標(biāo)識(shí)碼：A

文章編號(hào)：1007-3515(2002)01-0047-0052

基金項(xiàng)目：Supported by the National Natural Science Foundation of China(國(guó)家自然科學(xué)基金資助項(xiàng)目)

作者單位：閆培生（萊陽(yáng)農(nóng)學(xué)院應(yīng)用真菌研究室,山東萊陽(yáng),265200）　

　　　　　蔣家慧（萊陽(yáng)農(nóng)學(xué)院應(yīng)用真菌研究室,山東萊陽(yáng),265200）　

　　　　　王東昌（萊陽(yáng)農(nóng)學(xué)院應(yīng)用真菌研究室,山東萊陽(yáng),265200）　

　　　　　羅信昌（華中農(nóng)業(yè)大學(xué)農(nóng)業(yè)部農(nóng)業(yè)微生物重點(diǎn)實(shí)驗(yàn)室,武漢430070）　

　　　　　周啟（華中農(nóng)業(yè)大學(xué)農(nóng)業(yè)部農(nóng)業(yè)微生物重點(diǎn)實(shí)驗(yàn)室,武漢430070）　

參考文獻(xiàn)：

［1］Barrett M F, 1910. Three common species of Auricularia. Mycologia, 2: 12～18

［2］Berres M E, Szabo J, McLaughlin J, 1995. Phylogenetic relationships in auriculariaceous basidiomycetes based on 25S ribosomal DNA sequences. Mycologia, 87: 821～840

［3］Bidochka M J, McDonald M A, St-Leger R J, et al, 1994. Differentiation of species and strains of entomopathogenic fungi by random amplification of polymorphic DNA. Current Genetic,s 25: 107～ 113

［4］Boidin J, 1986. Intercompatibility and the species concept in the saprobic basidiomycotina. Mycotaxon, 26: 28～ 35

［5］Cheng S, Tu C C, 1978. Auricularia sp. The Biology and Cultivation of Edible Mushroom (ed.S.T.Chang, W.A.Hayes), 605～625 Academic Press: New York

［6］Guo Y R, Liu F C, Wang Y R, 1989. Biological characteristic of Auricularia maxima. Edible fungi, 5: 13～14. (In Chinese)

［7］Hibbett D S, Vilgalys R, 1991. Evolutionary relationships of Lentinus to the Polyporaceae: Evidence from restriction analysis of enzymatically amplified ribosomal DNA. Mycologia, 83: 425～439

［8］Hung C, 1983. A study on the morphology and inner structure of three Auricularia species cultivated in Taiwan. Taiwan Mushroom, 7: 1～13

［9］Kobayasi Y, 1942. On the genus Auricularia from eastern Asia. Bull Centr Natl Mus Manchoukuo, 4: 19～35

［10］Kobayasi Y, 1981. The genus Auricularia. Bull Natl Sci Mus B (Bot), 7: 41～6

［11］Li L J, 1984. Two new species of the genus Auricularia. Acta Mycologica Sinica, 4 (3): 149～154. (In Chinese.) )

［12］Lou L H, Zhu H Z, Tang H G, 1992. Preliminary study on the species of Auricularia. Journal of Edible fungi of China, 11(4):30～32.(In Chinese)

［13］Lowy B, 1951. A morphological basis for classifying the species of Auricularia. Mycologia, 43: 351～358

［14］Nicholson M, Bunyard B A, Royse D J, 1997. Phylogeny of the genus Lentinula based on ribosomal DNA restriction fragment Length polymorphism analysis. Mycologia, 89: 400～407

［15］Raper C A, Raper J R, Miller R E, 1972. Genetic analysis of the lifecycle of Agaricus bisporus. Mycologia, 74: 1088～1117

［16］Rohlf F J,1990. NTSYS-PC: numerical taxonomy and multivariate analysis system. Exeter Publishing: New York, U.S.A.

［17］Sambrook J, Fritsch E F, Maniatis T, 1989. Molecular cloning-A laboratory Manual.2nd ed. Cold spring Harbor Laboratory: Cold Spring Harbor, U.S.A.

［18］Wells K, 1994. Jelly fungi, then and now. Mycologia, 86: 18～48.

［19］Wong G J, Wells K, 1987. Comparative morphology, compatibility and interfertility of Auricularia cornea, A. polytrica and A.tenuis. Mycologia, 79: 847～856

［20］Wu Q X, 1992. Mating tests and systematics in higher basidiomycetes. Acta Mycologica Sinica, 11: 249～ 257. (In Chinese.)

［21］Yan P S, Luo X C and Zhou Q, 2000. Classification and identification of species and strains in Auricularia by using RAPD. Mycosystema, 19(1): 29～33. (In Chinese)

［22］Zhou Y L, Lu G Z, Liu W C, 1998. Systematic studies on Sphaeropsidales by using PCR-RFLP and RAPD analysis. Mycosystema, 17(2): 160～166.(In Chinese.)