【作者】劉建忠; 孫淑靜; 胡開輝; 熊芳; 肖雅敏; 陳明祥; 饒榆平; 張俊蘭

【Author】LIU Jian-zhong; SUN Shu-jing; HU Kai-hui; XIONG Fang; XIAO Ya-min; CHEN Ming-xiang; RAO Yu-ping; ZHANG Jun-lan(College of Life Sciences; Fujian Agriculture and Forestry University; Fuzhou Fujian 350002)

【作者機構】福建農林大學生命科學學院

【摘要】 運用原生質體融合與漆酶轉化體系篩選到一株真姬菇融合菌株(金山1號),研究了不同培養基配方、溫度、pH及含水量對該菌株菌絲生長的影響,并對該菌株的漆酶活性、生產特性進行了初步分析。結果表明,金山1號在PDA胡蘿卜培養基上菌絲生長最快、活力較強,菌絲最適生長溫度為25℃,最適pH為6.5,最適含水量60%～65%,漆酶活力為3.95 U·mL-1,生產周期可縮短3 d～5 d,生物學效率72.86%,與真姬菇相比提高了17.15%,表現出該融合菌株具有良好的生產應用價值。

【Abstract】 A Hypsizigus marmoreus fusion strain(Jinshan No.1) was screened by the technology of protoplast fusion and laccase transformation system.The effects of different culture media,temperature,pH and water content on the mycelial growth of fusion strain of Hypsizigus marmoreus were studied,and laccase activity and production characteristics of the fusion strain were preliminarily analysed.The experimental results showed that Jinshan No.1 strain had highest mycelial growth rate and the strongest mycelial growth vigor on the PDA carrot medium,and the optimum temperature,pH and water content were 25℃,pH 6.5,60%～65%.The laccase activity was 3.95 U·mL-1,and the biological efficiency was 72.86%,which increased 17.15% comparing with Hypsizigus marmoreus.The fruiting test results indicated that the fusion strain(Jinshan No.1) has good production value.

【關鍵詞】 真姬菇; 生物學特性; 融合子

【Key words】 Hypsizigus marmoreus; Biological characteristics; Fusion strain

【基金】福建省發改委農業“五新”推廣項目,編號:(2008)1119;; 福建省自然基金(2009J05046);; 福建省科技重點項目(2009N0010);; 福建省教育廳項目(JA09098);; 福建省教育廳項目(JA09097)

【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2010年03期

【DOI】CNKI:SUN:ZSYJ.0.2010-03-012

【分類號】S646.9