【作者】谷妲; 包海鷹

【Author】GU Da; BAO Hai-ying(Jilin Agriculture University; Changchun Jilin 130118)

【作者機構(gòu)】吉林農(nóng)業(yè)大學(xué)

【摘要】 采用顯微鑒定和理化檢識的方法對栽培樺褐孔菌和野生樺褐孔菌菌核進行生藥學(xué)的比較。結(jié)果顯示顯微鑒定中栽培與野生樺褐孔菌菌核差別在于前者菌絲松散,分枝較少;后者相反。理化檢識方面,薄層層析比較得到二者均含有白樺酯醇;GC-MS聯(lián)用分析鑒定出栽培樺褐孔菌菌核石油醚提取物中8個化合物,占總量的70.31%;野生樺褐孔菌菌核石油醚提取物中4個化合物,占總量的16.04%,并且二者沒有共同的化合物;高效液相色譜分析出栽培樺褐孔菌的粗水提取物和野生樺褐孔菌菌核的粗水提物在相同條件下,栽培樺褐孔菌菌核粗水提取物明顯的峰有2個,保留時間分別為85.026 min和107.920 min;野生樺褐孔菌菌核粗水提物明顯的峰有4個,保留時間分別為76.310 min、77.130 min、87.546 min和97.423 min?？梢钥闯?相同條件下二者比較,并未發(fā)現(xiàn)相同的成分。

【Abstract】 The comparison of pharmacognosy between cultivated and wild Inonotus obliquus sclerotium were analyzed by the microscopical identification and the methods of physical and chemical inspection knowledge.The differences between cultivated and wild Inonotus obliquus were insisted in some aspects in microscopical identification,such as the former had incompact hypha and less divarications,while the later was opposite.The betulin was the same compound in them by TLC.There were 8 compounds identified in petroleum ether layer of cultivated Inonotus obliquus(70.31%) by GC-MS,and there were 4 compounds identified in petroleum ether layer of wild Inonotus obliquus(16.04%) by GC-MS.There was no same compounds between them.The compare results between crud water extract of cultivated and wild Inonotus obliquus by HPLC under the same conditions showed that crude water extract of cultivated Inonotus obliquus had two obvious peaks,and retention time was 85.026 min and 107.920 min.Crude water extract of...更多 wild Inonotus obliquus had four obvious peaks,and the retention time respectively was 76.310 min,77.130 min,87.546 min,97.423 min.As can be seen,the same compounds were not found in crude water extracts of the cultivated and wild Inonotus obliquus under the same concentration.  還原

【關(guān)鍵詞】 栽培; 野生; 樺褐孔菌菌核; 顯微鑒定; 理化檢識

【Key words】 Cultivated; Wild; Inonotus obliquus sclerotium; Microscopical identification; Physical and chemical inspection knowledge

【基金】國家自然科學(xué)基金重大國際合作項目(編號:30510154);; 國家自然科學(xué)基金資助項目,項目批準號:30870013

【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2010年03期

【DOI】CNKI:SUN:ZSYJ.0.2010-03-019

【分類號】S567.39