【作者】汪虹; 陳明杰; 馮愛(ài)萍; 喬娜

【Author】WANG Hong; CHEN Mingjie; FENG Aiping; QIAO Na National Engineering Research Center of Edible Fungi; Key Laboratory of Applied Mycological Resources and Utilization; Ministry of Agriculture; Shanghai Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi; Shanghai Academy of Agricultural Sciences; 201106; P. R. China

【作者機(jī)構(gòu)】國(guó)家食用菌工程技術(shù)研究中心; 農(nóng)業(yè)部應(yīng)用真菌資源與利用重點(diǎn)開(kāi)放實(shí)驗(yàn)室; 上海市農(nóng)業(yè)遺傳育種重點(diǎn)開(kāi)放實(shí)驗(yàn)室; 上海市農(nóng)業(yè)科學(xué)院食用菌研究所

【摘要】 將草菇(Volvariella volvacea)菌株V23和耐低溫誘變菌株VH3的菌絲體在4℃下處理不同時(shí)間后,采用熒光定量PCR技術(shù)中的雙標(biāo)準(zhǔn)曲線法研究了S-腺苷-L-高半胱氨酸水解酶同源基因Cor3的表達(dá)變化。結(jié)果顯示,低溫處理后,草菇V23菌株的Cor3基因表達(dá)量隨處理時(shí)間的延長(zhǎng)呈不斷下降趨勢(shì),10h時(shí),其表達(dá)量?jī)H為對(duì)照的7%。而VH3菌株Cor3基因4℃處理2h表達(dá)量明顯上升,為對(duì)照的1.8倍,處理4h和6h表達(dá)量降低,分別為對(duì)照的80%和90%,處理8h的表達(dá)量又上升,為對(duì)照的1.4倍,隨后下降,處理10h表達(dá)量最低,為對(duì)照的60%。根據(jù)結(jié)果初步推測(cè)Cor3基因與草菇低溫耐受性具有一定相關(guān)性。

【Abstract】 The high temperatures necessary for vegetative growth and fruiting of the edible mushroom,Volvariella volvacea,restrict industrial scale cultivation to tropical and sub-tropical regions,thereby limiting the wider industrial exploitation of this important edible species. As part of a study aimed at developing commercially viable,cold-tolerant strains of V. volvacea capable of growing and fruiting in temperate climes,we have used real-time quantitative PCR to compare expression levels of a gene encoding for S-adenosyl-L-homocysteine hydrolase (Cor3) in two strains of V. volvacea,a low-temperature sensitive strain (V23) requiring temperatures >28 ℃ for normal vegetative growth and fruiting,and a cold-tolerant mutant (VH3),following exposure to 4 ℃. Relative expression levels of Cor3 in parental V23 mycelia exhibited a general decrease with time following exposure,declining by 80% and 30% of initial values after 2 h and 4 h,respectively. After 10 h,the transcription level was only 7% o...更多f that prior to low temperature exposure. Conversely,relative Cor3 expression levels in mutant VH3 mycelia exposed to 4 ℃ were proportionately higher throughout the treatment period. Expression levels after 2 h were 1.8-fold higher than initial levels,and then fluctuated between 80%-90% of zero time levels after 46 h. Gene transcription again increased (to 140% of zero time levels) after 8 h,and then fell to 60% of initial levels after 10 h exposure. Our data indicate that low levels of Cor3 expression are linked to the low temperature sensitivity of V. volvacea mycelium.  還原

【關(guān)鍵詞】 冷誘導(dǎo)基因表達(dá); 低溫耐受性; 實(shí)時(shí)定量PCR; S-腺苷-L-高半胱氨酸水解酶; 草菇

【Key words】 Cold-induced gene transcription; cold tolerance; real-time quantitative PCR; S-adenosyl-L-homocysteine hydrolase; Volvariella volvacea

【基金】上海農(nóng)科院2008年度院發(fā)基金[編號(hào):上海農(nóng)科院農(nóng)科發(fā)2008(16)];; 上海科技興農(nóng)重點(diǎn)攻關(guān)項(xiàng)目(編號(hào):滬農(nóng)科攻字2007第6-2號(hào));; 農(nóng)業(yè)部公益性行業(yè)科研專項(xiàng)項(xiàng)目(編號(hào):nyhyzx07-008)的部分研究?jī)?nèi)容~~

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2010年01期

【DOI】CNKI:SUN:SYJB.0.2010-01-005

【分類號(hào)】S646.13