【作者】孫曉紅; 陳明杰; 汪虹; 馮愛萍; 潘迎捷
【Author】SUN Xiaohong1; 2; 3; CHEN Mingjie1; WANG Hong1; FENG Aiping1; PAN Yingjie31National Engineering Research Center of Edible Fungi; Key Laboratory of Applied Mycological Resources and Utilization; Ministry of Agriculture; Shanghai Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi; Shanghai Academy of Agricultural Sciences; 201106; P. R. China; 2College of Life Sciences; Nanjing Agricultural University; Nanjing; Jiangsu 210095; China; 3Shanghai Ocean University; Shanghai 200090; China
【作者機構】國家食用菌工程技術研究中心; 農業部應用真菌資源與利用重點開放實驗室; 上海市農業遺傳育種重點開放實驗室; 上海市農業科學院食用菌研究所; 南京農業大學生命技術學院; 上海海洋大學
【摘要】 根據草菇(Volvariella volvacea)S-腺苷-L-高半胱氨酸水解酶基因(SAHH)全長cDNA序列設計引物,以草菇基因組DNA為模板,PCR擴增獲得草菇SAHH基因的全長序列,序列分析結果表明,草菇SAHH長度為1868bp,編碼430個氨基酸殘基,基因由12個外顯子和11個內含子組成,將草菇SAHH基因推斷的氨基酸序列與其它物種的SAHH基因氨基酸序列進行比較發現,SAHH基因保守性較高。
【Abstract】 The genomic sequence of a S-adenosyl-L-homocysteine hydrolase gene (SAHH) from Volvariella volvacea was amplified using specific primers designed according to the full-length SAHH cDNA. SAHH consists of 1 868 bp encoding 430 amino acids,and contains 12 exons and 11 introns. A phylogenetic tree constructed on the basis of the amino acid sequences of SAHH genes in V. volvacea and other reference species revealed a high degree of conservation.
【關鍵詞】 草菇; S-腺苷-L-高半胱氨酸水解酶基因; 克隆; 序列分析
【Key words】 Volvariella volvacea; S-adenosyl-L-homocysteine hydrolase gene; clone; sequence analysis
【基金】農業部公益性行業科研專項項目(編號:nyhyzx07-008);; 上海市科技興農重點攻關項目[編號:滬農科攻字(2007)第6-2號]的部分研究內容
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2010年01期
【DOI】CNKI:SUN:SYJB.0.2010-01-006
【分類號】S646.13