【作者】茆廣華; 徐財泉; 周曉芬; 蘭偉
【Author】MAO Guanghua; XU Caiquan; ZHOU Xiaofen; LAN Wei Zhejiang FangGe Pharmaceutical Co.; Ltd.; Qingyuan; Zhejiang 323800; China
【作者機構】浙江方格藥業有限公司
【摘要】 采用L9(34)正交試驗設計,考察灰樹花(Grifola frondosa)子實體粉碎粒度、料液比、提取時間和提取次數對粗多糖得率和樣品中總糖含量的影響;同時考察了不同種類和不同使用量的絮凝劑對灰樹花粗多糖中重金屬的去除效果。試驗結果表明,在4個考察因素中,原料粉碎粒度對兩個考察指標均有顯著影響,優化的水提工藝條件為超細粉(150目,2.8125μm),料液比1/30,提取時間1h,提取2次,此提取工藝條件下的粗多糖得率和樣品中總糖含量均較高;絮凝劑M(添加量為0.05%)對灰樹花粗多糖中Pb,As,Hg,Cd的去除率分別為76.6%,93.6%,>99.9%和97.9%,處理后的灰樹花多糖產品中重金屬含量符合出口要求。本試驗優化的提取工藝已用于灰樹花粗多糖生產,0.05%使用量的絮凝劑M可有效去除粗多糖產品中的重金屬。
【Abstract】 Orthogonal testing [L9(34)] was used to optimize selected parameters affecting polysaccharide extraction from Grifola frondosa fruit bodies that had been dried and pulverised. Particle size was the most influential parameter,and the optimal extraction conditions were:particle size 2.8125 μm,solid-liquid ratio 1/30,extraction time 2 h,and three successive extractions. Of several treatments evaluated,0.05% Flocculant M was most effective in lowering the heavy metal content of extracted polysaccharide,removing 76.6%,93.6%,>99.9% and 97.9% of the Pb,As,Hg and Cd residues,respectively.
【關鍵詞】 灰樹花; 多糖; 提取工藝優化; 重金屬去除
【Key words】 Grifola frondosa; crude polysaccharides; optimization of extraction conditions; heavy metal removal
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2010年01期
【DOI】CNKI:SUN:SYJB.0.2010-01-018
【分類號】S567.39