張紅玉; 付立忠; 吳學謙; 李海波; 魏海龍; 吳慶其; 王立安
河北師范大學生命科學學院; 浙江省林業科學研究院; 浙江省森林資源生物與化學利用重點實驗室; 浙江省林業科學研究院浙江省森林資源生物與化學利用重點實驗室; 麗水市食用菌研究開發中心; 浙江益圣菌物發展有限公司
【中文摘要】 為驗證新型SRAP分子標記技術用于靈芝(Ganoderma lucidum)原生質體融合子鑒定的可行性,通過親本菌株赤芝05、06對153個SRAP引物組合進行篩選,應用經過初篩和復篩得到的4個清晰穩定、且含有兩個親本各自特異擴增條帶的SRAP引物組合,對2個親本菌株、2個只與親本05有拮抗的融合子、2個只與親本06有拮抗的融合子、30個與雙親均有拮抗的融合子進行SRAP擴增。結果表明,2個只與親本05有拮抗的融合子僅擴增出06的特異條帶,2個只與親本06有拮抗的融合子僅擴增出05的特異條帶,30個與雙親均有拮抗的融合子中,有7個融合子同時擴增出05和06的特異條帶,證明此7個融合子應是05和06融合產生的菌株,也證明SRAP標記可以用于靈芝原生質體融合子的鑒定。
【英文摘要】 SRAP makers were used to identify protoplast fusion hybrids of Ganoderma lucidum.A total of 153 SRAP primer combinations were selected using G.lucidum parental strains 05 and 06.Four primer pairs generated clear,stable and specific bands for each of the parental strains,and were used to confirm protoplast fusion hybrids.A total of 181 strains were isolated from regenerated protoplasts after fusion treatment of equal numbers of protoplasts prepared from each of the two parental strains.These strains were divided into three groups based on their antagonistic effect with the two parental strains: 35 isolates exhibited antagonism with parental strain 05(Group A),116 isolates with parental strain 06(Group B),and 30 isolates with both parental strains(Group C).SRAP amplification was then carried out with the two parental strains,with two isolates each from A and B groups,and with all the Group C isolates using the four selected SRAP primer pairs.Bands specific only for parental strain 06 wer...更多e observed with Group A isolates,whereas Group B isolates generated bands specific only for parental strain 05.However,bands specific for both parental strains were observed with seven Group C isolates,thereby confirming these isolates to be true protoplast fusion hybrids. 還原
【中文關鍵詞】 藥用菌; 靈芝; SRAP分子標記; 原生質體融合; 雜種鑒定
【英文關鍵詞】 Medicinal mushrooms; Ganoderma lucidum; SRAP molecular marker; protoplast fusion; hybrid identification
【基金】浙江省科研農業項目“食用菌雜交育種親本選擇及雜交子分子鑒定技術研究”(編號:2007C22069);; 浙江省青年人才培養項目“食藥用真菌種質資源遺傳評價及DNA分子標記鑒定技術研究”(編號:2006R20006);; 浙江省創新團隊建設與人才培養項目“現代食用菌科技創新團隊建設”;; 浙江省森林資源生物與化學利用重點實驗室項目“高多糖、高三萜含量香菇、靈芝種質創制與菌株篩選”的部分研究內容
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2009年04期 【DOI】CNKI:SUN:SYJB.0.2009-04-001