杜秀菊; 潘迎捷; 張勁松; 唐慶九; 楊焱; 賈薇; 劉艷芳
國家食用菌工程技術研究中心農業部應用真菌資源與利用重點開放實驗室上海市農業遺傳育種重點實驗室上海市農業科學院食用菌研究所上海市農業科學院天然藥用資源研究開發中心; 聊城大學生命科學學院; 上海海洋大學
【中文摘要】 采用體外腫瘤細胞株(小鼠白血病細胞株L1210、人乳腺癌細胞株MCF-7和人腸腺癌細胞株SW620)的增殖抑制試驗模型和抗病毒(流感甲型病毒和皰疹病毒)的篩選模型,對3個金耳(Tremella aurantialba)菌株菌絲體醇提物活性進行比較研究。抗腫瘤試驗結果表明,3種提取物材料在高濃度(200μg/mL)時,對3個供試腫瘤細胞株的細胞增殖均有明顯的抑制作用,其中以YK的抑制活性最高(對L1210、SW620和MCF-7的抑制率分別為89.4%,75.0%和64.1%);在中濃度(50μg/mL)時,均表現出一定的抑制作用;而在低濃度(10μg/mL)時,則均無明顯的抑制作用。抗流感甲型病毒和抗皰疹病毒試驗結果表明,只有YK對流感甲型病毒表現有抗病毒活性,但此活性較弱。
【英文摘要】 Ethanolic extracts of mycelia from three strains of Tremella aurantialba were tested for in vitro tumor cell inhibitory effects using murine leukemia(L1210),human intestinal adenocarcinoma(SW620) and human breast cancer(MCF-7) cell lines,and for antiviral activity using A-FLU,HSV-Ⅰand HSV-Ⅱviral models.Significant inhibition of tumor cell proliferation by extracts of all three mycelial samples was observed at 200 μg/mL concentration.Highest levels of L1210,SW620 and MCF-7 cell inhibition,observed with extracts of strain YK,were 89.4%,75.0% and 64.1%,respectively.Lower inhibitory effects were recorded at 50 μg/mL concentrations,and only weak inhibition was observed when cell lines were treated with extract concentrations of 10 μg/mL.Antiviral activity,in the form of weak effects against A-FLU virus,was observed only in the case of ethanolic extracts of strain YK mycelium.
【中文關鍵詞】 金耳; 醇提物; 抗腫瘤; 抗病毒
【英文關鍵詞】 Tremella aurantialba; ethanol extracts; antitumor activity; antiviral activity
【基金】國家科技部自然科學基金項目(編號:2006BAD06B08)的部分研究內容
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2009年04期 【DOI】CNKI:SUN:SYJB.0.2009-04-009