張美彥; 尚曉冬; 李玉; 郭倩; 陳明杰; 譚琦
農(nóng)業(yè)部應(yīng)用真菌資源與利用重點(diǎn)開放實(shí)驗(yàn)室上海市食用菌工程技術(shù)研究中心上海市農(nóng)業(yè)遺傳育種重點(diǎn)實(shí)驗(yàn)室上海市農(nóng)業(yè)科學(xué)院食用菌研究所
【中文摘要】 以25個(gè)灰樹花(Grifola frondosa)菌株的子實(shí)體為材料提取基因組DNA,ITS擴(kuò)增后對產(chǎn)物進(jìn)行酶切,25個(gè)菌株的ITS條帶處于同一位置,酶切后部分菌株呈現(xiàn)不一樣的切點(diǎn),可將這些菌株大致分為三類。應(yīng)用擴(kuò)增清晰、多態(tài)性高的47對SRAP引物對這25個(gè)菌株進(jìn)行SRAP分析,共得到138條多態(tài)性條帶,樹狀圖顯示,這些菌株在相似度77%水平上可分為三類,與ITS-RFLP的分析結(jié)果類似。SRAP分析結(jié)果顯示,有10個(gè)菌株可以被有效的區(qū)分,15個(gè)菌株未能找到其相互之間的差異帶,說明ITS-RFLP和SRAP標(biāo)記可以應(yīng)用于區(qū)分菌株,進(jìn)行種質(zhì)評(píng)價(jià)。
【英文摘要】 Genomic DNA samples prepared from fruit bodies of 25 Grifola frondosa strains were amplified with ITS(internal transcribed spacer)primers.Although the PCR reaction products exhibited identical electrophoresis patterns,hydrolysis using a range of restriction endonucleases revealed that the ITS bands contained different restriction enzyme cutting sites and could be separated into three broad groups based on the sequences of the hydrolytic products.SRAP amplification employing 47 SRAP(sequence related amplified polymorphism) primer pairs generated 138 polymorphic bands.A phylogram constructed using the SRAP data effectively differentiated ten strains whereas no obvious differences were detected between the remaining 15 strains.Our data implied that ITS-RFLP and SRAP markers were effective in G.frondosa strain identification and germplasm evaluation.
【中文關(guān)鍵詞】 灰樹花; SRAP; ITS; 種質(zhì)評(píng)價(jià)
【英文關(guān)鍵詞】 Grifola frondosa; sequence related amplified polymorphism(SRAP); internal transcribed spacer(ITS); germplasm evaluation
【基金】上海市科技興農(nóng)重點(diǎn)攻關(guān)項(xiàng)目“食用菌工廠化生產(chǎn)關(guān)鍵技術(shù)研究”[滬農(nóng)科攻字(2006)第3-1號(hào)]的部分研究內(nèi)容
【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2009年03期 【DOI】CNKI:SUN:SYJB.0.2009-03-002