張美彥; 尚曉冬; 李玉; 郭倩; 陳明杰; 譚琦
農業部應用真菌資源與利用重點開放實驗室上海市食用菌工程技術研究中心上海市農業遺傳育種重點實驗室上海市農業科學院食用菌研究所
【中文摘要】 以25個灰樹花(Grifola frondosa)菌株的子實體為材料提取基因組DNA,ITS擴增后對產物進行酶切,25個菌株的ITS條帶處于同一位置,酶切后部分菌株呈現不一樣的切點,可將這些菌株大致分為三類。應用擴增清晰、多態性高的47對SRAP引物對這25個菌株進行SRAP分析,共得到138條多態性條帶,樹狀圖顯示,這些菌株在相似度77%水平上可分為三類,與ITS-RFLP的分析結果類似。SRAP分析結果顯示,有10個菌株可以被有效的區分,15個菌株未能找到其相互之間的差異帶,說明ITS-RFLP和SRAP標記可以應用于區分菌株,進行種質評價。
【英文摘要】 Genomic DNA samples prepared from fruit bodies of 25 Grifola frondosa strains were amplified with ITS(internal transcribed spacer)primers.Although the PCR reaction products exhibited identical electrophoresis patterns,hydrolysis using a range of restriction endonucleases revealed that the ITS bands contained different restriction enzyme cutting sites and could be separated into three broad groups based on the sequences of the hydrolytic products.SRAP amplification employing 47 SRAP(sequence related amplified polymorphism) primer pairs generated 138 polymorphic bands.A phylogram constructed using the SRAP data effectively differentiated ten strains whereas no obvious differences were detected between the remaining 15 strains.Our data implied that ITS-RFLP and SRAP markers were effective in G.frondosa strain identification and germplasm evaluation.
【中文關鍵詞】 灰樹花; SRAP; ITS; 種質評價
【英文關鍵詞】 Grifola frondosa; sequence related amplified polymorphism(SRAP); internal transcribed spacer(ITS); germplasm evaluation
【基金】上海市科技興農重點攻關項目“食用菌工廠化生產關鍵技術研究”[滬農科攻字(2006)第3-1號]的部分研究內容
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2009年03期 【DOI】CNKI:SUN:SYJB.0.2009-03-002