李浩華; 陳玉嬋; 陶美華; 李冬利; 潘清靈; 王磊; 章衛(wèi)民
廣東省微生物研究所廣東省菌種保藏與應(yīng)用重點(diǎn)實(shí)驗(yàn)室廣東省微生物應(yīng)用新技術(shù)公共實(shí)驗(yàn)室
【中文摘要】 以人肺癌細(xì)胞NCI-H460、中樞神經(jīng)系統(tǒng)癌細(xì)胞SF-268及乳腺癌細(xì)胞MCF-7為供試細(xì)胞株,采用MTT(四甲基偶氮唑鹽)法對毛蜂窩菌(Hexagona apiaria)野生子實(shí)體、栽培子實(shí)體、培養(yǎng)廢料、發(fā)酵菌絲體的醇提物和發(fā)酵液乙酸乙酯萃取物、發(fā)酵液水相部分等各提取物進(jìn)行了體外抗腫瘤活性研究。結(jié)果表明,毛蜂窩菌發(fā)酵液乙酸乙酯萃取物對三種腫瘤細(xì)胞株都有較明顯的抑制作用,抑制率達(dá)80%以上;其它提取物也有一定的抑制作用,但抑制率在40%以下。
【英文摘要】 The anti-tumor activities of Hexagona apiaria extracts were evaluated with NCI-H460,SF-268 and MCF-7 cell lines using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium(MTT) method.Ethyl acetate extracts of the spent submerged culture medium significantly inhibited(>80%) all three tumor cell lines.Inhibitory effects were also observed with ethanolic extracts of both wild and artificially cultivated fruit bodies,spent compost after artificial cultivation,and fungal mycelium grown in submerged culture,as well as the aqueous phase remaining after ethyl acetate extraction of spent submerged culture medium,although the level of inhibition was <40% in all cases.
【中文關(guān)鍵詞】 毛蜂窩菌; 提取物; 抗腫瘤活性; MTT法
【英文關(guān)鍵詞】 Hexagona apiaria; extract; anti-tumor activity; MTT
【基金】廣東省科技計(jì)劃重點(diǎn)項(xiàng)目“食用菌產(chǎn)業(yè)推進(jìn)關(guān)鍵技術(shù)研究與示范”(編號:2009B020201012)的部分研究內(nèi)容
【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2009年03期 【DOI】CNKI:SUN:SYJB.0.2009-03-014