李浩華; 陳玉嬋; 陶美華; 李冬利; 潘清靈; 王磊; 章衛民
廣東省微生物研究所廣東省菌種保藏與應用重點實驗室廣東省微生物應用新技術公共實驗室

【中文摘要】 以人肺癌細胞NCI-H460、中樞神經系統癌細胞SF-268及乳腺癌細胞MCF-7為供試細胞株,采用MTT(四甲基偶氮唑鹽)法對毛蜂窩菌(Hexagona apiaria)野生子實體、栽培子實體、培養廢料、發酵菌絲體的醇提物和發酵液乙酸乙酯萃取物、發酵液水相部分等各提取物進行了體外抗腫瘤活性研究。結果表明,毛蜂窩菌發酵液乙酸乙酯萃取物對三種腫瘤細胞株都有較明顯的抑制作用,抑制率達80%以上;其它提取物也有一定的抑制作用,但抑制率在40%以下。

【英文摘要】 The anti-tumor activities of Hexagona apiaria extracts were evaluated with NCI-H460,SF-268 and MCF-7 cell lines using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium(MTT) method.Ethyl acetate extracts of the spent submerged culture medium significantly inhibited(>80%) all three tumor cell lines.Inhibitory effects were also observed with ethanolic extracts of both wild and artificially cultivated fruit bodies,spent compost after artificial cultivation,and fungal mycelium grown in submerged culture,as well as the aqueous phase remaining after ethyl acetate extraction of spent submerged culture medium,although the level of inhibition was <40% in all cases.

【中文關鍵詞】 毛蜂窩菌; 提取物; 抗腫瘤活性; MTT法
【英文關鍵詞】 Hexagona apiaria; extract; anti-tumor activity; MTT
【基金】廣東省科技計劃重點項目“食用菌產業推進關鍵技術研究與示范”(編號:2009B020201012)的部分研究內容

【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2009年03期 【DOI】CNKI:SUN:SYJB.0.2009-03-014