沈學香; 尚曉冬; 汪虹; 譚琦
農業部應用真菌資源與利用重點開放實驗室; 上海市食用菌工程技術研究中心; 上海市農業遺傳育種重點實驗室; 上海市農業科學院食用菌研究所
【中文摘要】 用對峙培養技術在PDA平板上對10株香菇菌株的營養體不親合性進行了研究。結果表明,供試菌株的營養體不親合性表現可分為兩種不親合類型和一種親合類型。在2次重復實驗中表現完全一致的有48對組合,占87.3%;表現不同但仍可認為是親合類型而不影響結果性質的有4例,占7.3%,分別是135×66、9015×66、9015×7402和Cr04×野生50;表現出相反的結果,影響親合性判定的有3例占5.4%,分別是Cr04×26、Cr04×申香10號和26×蘇香。11株供試香菇菌株間營養體不親合性的表現以不親合類型為主,第一種和第二種不親合類型分別出現86次(78.2%)和13次(11.8%);親合類型(第三種類型)較少,有11次(10%)。135、939和9015間彼此親合,它們與其它菌株均表現不親合。申香10號與26親合,2菌株與除Cr04和蘇香以外的菌株均不親合。
【英文摘要】 Somatic incompatibility (SI) between ten cultivars and one wild-type strain of Lentinula edodes was determined from mycelial growth confrontation reactions on potato dextrose agar plates. Two types of SI reactions were observed, together with one type of somatic compatibility (SC) reaction. Identical SI or SC reactions were recorded in duplicate confrontation tests between 48 pairs of L. edodes strains yielded, accounting for 87.3% of the total 110 test pairings. Different SI reactions were observed in duplicate pairings between strains 135 and 66, 9015 and 66, 9015 and 7402, and Cr04 and 50 (7.3% of the total). The opposite reaction (from SI to SC or vice versa) was observed in three sets of duplicate confrontation tests (5.4% of the total pairings) involving strains Cr04 and 26, Cr04 and Shenxiang 10, and 26 and Suxiang. The two SI reaction types were the main responses between the eleven test strains and accounted for 90% of the total. Strains 9015, 135 and 939 were somatically com...更多patible with each other but somatic incompatible with the remaining strains. Strains Shenxiang 10 and 26 were somatically compatible with each other but somatically incompatible with all the other strains except Cr04 and Suxiang. 還原
【中文關鍵詞】 香菇; 營養體不親合性; 拮抗反應
【英文關鍵詞】 Lentinula edodes; somatic incompatibility; antagonism reaetion
【基金】上海市農委科技興農重點攻關“香菇純‘自交系’育種技術的應用研究”的部分研究內容
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2009年02期 【DOI】CNKI:SUN:SYJB.0.2009-02-001