陳瑞鵬; 賈小寧; 郭立忠
青島農(nóng)業(yè)大學(xué)生命科學(xué)學(xué)院山東省應(yīng)用真菌重點(diǎn)實(shí)驗(yàn)室

【中文摘要】 采用RAPD、SRAP兩種標(biāo)記方法分別對(duì)6個(gè)繡球菌(Sparassis crispa)菌株進(jìn)行親緣關(guān)系分析,結(jié)果分析表明2種方法結(jié)果一致,平均每條引物組合產(chǎn)生的條帶數(shù)分別為20.5和24.5,多態(tài)性條帶的比例分別為91.4%和78.8%。結(jié)果顯示,6個(gè)菌株間均有遺傳上的差異,菌株4號(hào)、5號(hào)同源性最大,相似系數(shù)分別達(dá)到了0.981和0.875,說(shuō)明菌株4號(hào)、5號(hào)可能是同物異名。

【英文摘要】 Six strains of Sparassis crispa conserved by Agricultural Applied Mycology Lab, Qingdao Agricultural University, were studied by using the molecular techniques of RAPD and SRAP in this experiment. The results showed that the results of two methods are consistent. Each primer combination generated the average strip of 20.5 and 24.5, respectively. The proportion of polymorphic bands were 91.4% and 78.8% respectively, and 6 strains were different at genetic. Sparassis crispa strain 4 and 5 exhibited a higher genetic similarity (similar coefficients were up to 0.9813 and 0.8570), which showed close phylogenetic relationship.

【中文關(guān)鍵詞】 繡球菌; RAPD; SRAP; 聚類分析
【英文關(guān)鍵詞】 Sparassis crispa; RAPD; SRAP; Cluster analysis
【基金】國(guó)家支撐項(xiàng)目,黃淮平原區(qū)農(nóng)田秸稈資源綜合利用技術(shù)集成研究與示范(2007BAD89B09-15)

【文獻(xiàn)出處】 中國(guó)食用菌,Edible Fungi of China,編輯部郵箱,2010年02期 【DOI】CNKI:SUN:ZSYJ.0.2010-02-015