李翠翠; 尉玉曉; 郭立忠
青島農業大學生命科學學院農業應用真菌研究室
【中文摘要】 實驗研究了桑黃液體發酵培養基中不同營養組成對桑黃菌絲生長的影響。以菌絲生物量和多糖產量為主要指標,對桑黃液體發酵培養基進行了優化。通過單因素試驗和正交試驗篩選出了桑黃液體發酵的最佳培養基。單因素試驗結果表明最適氮源為麥麩,最佳碳氮比為20:1;正交試驗結果表明,優化培養基配方為玉米粉50g·L-1、麥麩50g·L-1、KH2PO42g·L-1、MgSO41g·L-1、VB1300μg·L-1、VB2400μg·L-1。
【英文摘要】 The effects of cultural compounds on the growth of Phellinus igniarius mycelium were investigated.Taking the mycelial biomass and production of polysaccharide as detecting indices,the optimum submerged fermentation parameters for Phellinus igniarius were studied by single factor test and orthogonal test.The result by single factor test showed that the optimum nitrogen source was bran and C/N was 20:1.Through orthogonal test,the optimum formula for submerged fermentation were proposed of maize powder 50 g·L-1,bran 50 g·L-1,KH2PO4 2 g·L-1,MgSO4 1 g·L-1,VB1 300 μg·L-1,VB2 400 μg·L-1.
【中文關鍵詞】 桑黃; 液體發酵; 生物量; 多糖
【英文關鍵詞】 Phellinus igniarius; Liquid fermentation; Biomass; Polysaccharide
【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2009年02期 【DOI】CNKI:SUN:ZSYJ.0.2009-02-017