楊永彬; 林遠崇; 蘭家細; 楊淑云; 羿紅
福建省蠶桑研究所; 福建省蠶桑研究所; 福建省蠶桑研究所; 福建省蠶桑研究所; 福建省蠶桑研究所 福建福州350003; 福建福州350003; 福建福州350003; 福建福州350003; 福建福州350003

【中文摘要】 根據(jù)真菌核糖體通用引物ITS1和ITS4擴增出13個福建袋栽香菇主要菌株的ITS、5.8s rDNA序列,將該序列提交NCBI中Genbank數(shù)據(jù)庫,并根據(jù)該序列特征及ITS區(qū)的差異性,分別設計出針對菌株L9015和菌株Cr02進行PCR檢測的特異引物探針,結(jié)果顯示特異引物具有良好的特異性。

【英文摘要】 ITS(Internal-transcribed sequence) reigon has been often used to identify and characterize the microorganisms due to its high rate of mutation during the process of evolution.In this paper,the universe eukaryotic ribosome primers ITS1&ITS4 are used to amplify the ITS5.8s rDNA in the genome of thirteen Lentinula edodes strains,and then sequence these ITS5.8s rDNA sequences.After a careful analysis and comparison among them,two pairs of PCR primers(designated pair primer 1 and pair primer 2)are designed which can be served as the molecular marker to specificly amplify one piece of ITS5.8s rDNA from strain L9015 and strain Cr02.Result shows one piece of DNA(around 540bp in length and aonther(180bp) are specificly amplified from strain L9015 and strain Cr02 respecitively.

【中文關(guān)鍵詞】 香菇; 菌株; ITS5.8srDNA; 特異引物
【英文關(guān)鍵詞】 Lentinula edodes; Strain; ITS5.8srDNA; Specific PCR primer
【基金】福建省自然科學基金(B0510036)

【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2008年02期 【DOI】CNKI:SUN:ZSYJ.0.2008-02-015