凱氏定氮法測定靈芝破壁孢子粉中粗蛋白質(zhì)含量的不確定度評定
Evaluation of the Uncertainty Associated with the Measurement of Protein in Ganoderma lucidum Wall-broken Spores Using the Kjeldahl Method

【作者】 王紅梅； 雷萍； 王少鷗； 劉海燕；

【Author】 WANG Hongmei,LEI Ping,WANG Shaoou,LIU Haiyan Institute for Agro-Food Standards and Testing Technology,Shanghai Academy of Agricultural Sciences, Supervision and Testing Center for Edible Fungi Quality at Shanghai,Ministry of Agriculture, Shanghai 201403,China

【機(jī)構(gòu)】 上海市農(nóng)業(yè)科學(xué)院農(nóng)產(chǎn)品質(zhì)量標(biāo)準(zhǔn)與檢測技術(shù)研究所,農(nóng)業(yè)部食用菌產(chǎn)品質(zhì)量監(jiān)督檢驗(yàn)測試中心(上海)；

【摘要】 對采用凱氏定氮法測定靈芝(Ganoderma lucidum)破壁孢子粉中粗蛋白含量的不確定度進(jìn)行評定,結(jié)果表明合成相對標(biāo)準(zhǔn)不確定度為0.02%,擴(kuò)展不確定度為0.04%。在本次孢子粉粗蛋白質(zhì)測定中,引入不確定度的主要因素有鹽酸標(biāo)準(zhǔn)溶液的濃度及樣品測定中實(shí)際消耗的標(biāo)準(zhǔn)溶液體積。

【Abstract】 We evaluated the level of uncertainty associated with the measurement of crude protein in Ganoderma lucidum wall-broken spores using the Kjeldahl method.Our data revealed that the combined related standard uncertainty and the expanded uncertainty values were 0.02% and 0.04%,respectively.The level of uncertainty associated with the measurement of crude protein in G.lucidum wall-broken spores is mainly determined by the concentration of the standard hydrochloric acid solution and the volume of aci...

【關(guān)鍵詞】 凱氏定氮法； 靈芝破壁孢子粉； 粗蛋白質(zhì)含量； 不確定度評定；
【Key words】 Kjeldahl method； Ganoderma lucidum wall-broken spores； crude protein content； uncertainty evaluation；

【分類號】TS219