周長青; 王秀峰; 李玉
山東農業大學園藝科學與工程學院; 吉林農業大學

【中文摘要】 以白靈菇(Pleurotus nebrodensis)天山2號菌株為試材,研究了白靈菇生長發育過程中胞外濾紙酶、羧甲基纖維素酶、半纖維素酶、淀粉酶、漆酶、酸性蛋白酶和中性蛋白酶活性變化的規律。結果表明,整個生育期的胞外酶活性變化具有明顯的階段性。濾紙酶、羧甲基纖維素酶和半纖維素酶活性菌絲生長階段較低,現蕾后活性急劇增強。酸性蛋白酶和中性蛋白酶活性菌絲生長階段比較穩定,現蕾前相對較低,子實體生長階段兩種酶的活性又明顯增強。整個生長階段酸性蛋白酶的活性明顯高于中性蛋白酶的活性。

【英文摘要】 Changes in the levels of seven extracellular enzymes(filter paper enzyme,carboxymethyl cellulase,hemicellulase,amylase,laccase,acid proteinase and neutral proteinase) were recorded at different stages of the developmental cycle of Pleurotus nebrodensis,strain"Tianshan No.2".Filter paper enzyme,carboxymethyl cellulase and hemicellulase levels were relatively low during the vegetative growth phase but increased sharply at the onset of primordium formation.Acid and neutral proteinase activities remained constant during vegetative growth,decreased to relatively low levels before the appearance of primordia,and increased again during the fruit body growth stage.

【中文關鍵詞】 白靈菇; 生長發育; 濾紙酶; 羧甲基纖維素酶; 半纖維素酶; 淀粉酶; 漆酶; 蛋白酶
【英文關鍵詞】 Pleurotus nebrodensis; growth and development; filter paper enzyme; carboxymethyl cellulase; hemicellulase; amylase; laccase; proteinase

【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2008年02期 【DOI】CNKI:SUN:SYJB.0.2008-02-016