韓增華; 張丕奇; 孔祥輝; 馬慶芳; 戴肖東; 張介馳
黑龍江省科學院應用微生物研究所; 黑龍江省科學院應用微生物研究所; 黑龍江省科學院應用微生物研究所; 黑龍江省科學院應用微生物研究所; 黑龍江省科學院應用微生物研究所; 黑龍江省科學院應用微生物研究所 哈爾濱150010; 哈爾濱150010; 哈爾濱150010; 哈爾濱150010; 哈爾濱150010; 哈爾濱150010

【中文摘要】 研究了黑木耳10個菌株胞外酶活性及與栽培性狀和產量的關系,結果表明,胞外漆酶、多酚氧化酶活性的變化趨勢大致相似,酶活與栽培產量呈正相關,相關系數為0.823(7號菌株除外),酶活性相對較高的菌株(3、4、8號),栽培中產量相對較高。纖維素酶活性高峰時間出現較漆酶、多酚氧化酶早,且酶活性高峰出現早的菌株菌絲長速較快。蛋白酶活與栽培產量呈一定負相關性,相關系數為-0.700(6號菌株除外),蛋白酶活最低的3號菌株,栽培產量最高;而活性較高的5、9號菌株栽培產量較低。

【英文摘要】 Production of selected extracellular enzymes [laccase,polyphenoloxidase(PPO),carboxymethyl cellulase(CMCase),filter paper cellulase(FPase)and acid protease] by 10 strains of Auricularia auricula was determined during growth in submerged culture.Similar patterns of laccase and PPO production were observed during the 49-day culture period and,apart from strain No.7,a positive relationship(correlation coefficient 0.823)was observed between both these enzyme activities and fruit body yields obtained following cultivation of the different strains on a sawdust-wheat bran substrate.Strains.3,4 and 8,that exhibited relatively higher laccase and PPO activities,produced higher fruit body yields.Peak cellulase activities were recorded earlier compared with laccase and PPO,and the mycelia of strains attaining the earliest peak enzyme levels grew faster.Except in the case of strain No.6,there was a negative correlation(correlation coefficient 0.70)between protease activity and fruit body yields Str...更多ains 5 and 9 with the highest protease activities had the lowest yields,while strain 3 with the lowest protease activity produced the highest yield.  還原

【中文關鍵詞】 黑木耳; 胞外酶活性; 菌絲長速; 液體培養; 子實體產量; 袋栽
【英文關鍵詞】 Auricularia auricula; Extracellular enzyme activity; Mycelial growth rate; Submerged culture; Fruit body yield ; Bag cultivation
【基金】黑龍江省科學院項目“黑木耳菌種早期性狀鑒定研究”(編號01BFCAHZH10)的部分研究內容

【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2007年04期 【DOI】CNKI:SUN:SYJB.0.2007-04-011