林新堅(jiān); 江秀紅; 林戎斌; 陳濟(jì)琛
福建省農(nóng)業(yè)科學(xué)院土壤肥料研究所; 福建師范大學(xué)生命科學(xué)學(xué)院; 福建省農(nóng)業(yè)科學(xué)院土壤肥料研究所; 福建省農(nóng)業(yè)科學(xué)院土壤肥料研究所 福州350013; 福州350108; 福州350013; 福州350013
【中文摘要】 研究了姬松茸(Agaricus blazei Murrill)菌株(出發(fā)菌株AbM9,AbM9經(jīng)離子束注入選育的菌株AbML2、AbML7和AbML11,及AbM9單孢分離所得菌株AbMD3)在菌絲生長期、菇蕾期、一潮菇期和二潮菇期培養(yǎng)料的胞外羧甲基纖維素酶、淀粉酶和蛋白酶活性的動態(tài)變化及與產(chǎn)量的關(guān)系。試驗(yàn)結(jié)果表明,姬松茸菌株AbML11產(chǎn)量最高,與AbML2、AbMD3、AbML7和AbM9的差異達(dá)極顯著水平,但AbML11的產(chǎn)量與菌絲長速相關(guān)性不顯著;5個供試菌株的淀粉酶活性在菌絲生長期均最高,與菌絲長速的相關(guān)性不顯著;蛋白酶活性在菇蕾期最高,與產(chǎn)量的相關(guān)性不顯著;羧甲基纖維素酶活性在一潮菇期最高,與產(chǎn)量呈正相關(guān),相關(guān)系數(shù)為0.928,達(dá)顯著水平。本研究可為姬松茸高產(chǎn)菌株的篩選提供一定的理論依據(jù)。
【英文摘要】 Variations in carboxymethyl cellulase (CMCase), amylase and protease production by Agaricus blazei Murill strains AbM9 (parent strain), AbMD3 (derived from a single spore isolated from a fruit body of AbM9), AbML2, AbML7 and AbML11 (these latter three strains were induced by ion beam mutation of AbM9) during mycelial growth, primordium formation, and the first and second flush periods were correlated with biological efficiency. Of the five test strains, highest yields were obtained with AbML11. In all five strains, amylase production reached maximal levels during the mycelial growth stage and gradually decreased thereafter, whereas protease levels peaked during the period of primordium formation. Peak levels of CMCase coincided with the first flush of fruit bodies, and there was a strong positive correlation (0.928) between CMCase activity and biological efficiency. These data should facilitate the development of strategies aimed at selecting high yield strains of this mushroom.
【中文關(guān)鍵詞】 姬松茸; 胞外酶; 纖維素酶; 生物學(xué)效率
【英文關(guān)鍵詞】 Agaricus blazei Murill; Extracellular enzyme; CMCase; Biological efficiency
【基金】福建省科技廳項(xiàng)目“姬松茸低鎘品種選育”(編號2002I010);; 福建省財(cái)政專項(xiàng)“福建省農(nóng)業(yè)科學(xué)科技創(chuàng)新團(tuán)隊(duì)建設(shè)基金”(STIF-Y01)的部分研究內(nèi)容
【文獻(xiàn)出處】 食用菌學(xué)報,Acta Edulis Fungi,編輯部郵箱,2007年03期 【DOI】CNKI:SUN:SYJB.0.2007-03-007