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  • Construction of a Plasmid and a Standard Curve for Real-time Quantitative PCR of the Cold-induced Co


    【發(fā)布日期】:2010-05-17

    WANG Hong; CHEN MingjieKey Laboratory of Agricultural Genetics and Breeding of Shanghai; Institute of Edible Fungi; Shanghai Academy of Agricultural Sciences; Shanghai 201106; China

    【中文摘要】 A plasmid containing target DNA and a standard curve for real-time quantitative PCR of the cold-induced Cor3 gene of Volvariella volvacea were constructed. These will provide the basis for further research on Cor3 gene expression at low temperature, and ultimately for assigning a role for the gene in the low temperature autolysis of V. volvacea.

    【英文摘要】 A plasmid containing target DNA and a standard curve for real-time quantitative PCR of the cold-induced Cor3 gene of Volvariella volvacea were constructed. These will provide the basis for further research on Cor3 gene expression at low temperature, and ultimately for assigning a role for the gene in the low temperature autolysis of V. volvacea.

    【英文關(guān)鍵詞】 Volvariella volvacea; Cold induction; Cor3 gene; Quantitative PCR; Plasmid; Standard curve
    【基金】Sponsored by the Shanghai Academy of Agricultural Science and Shanghai Science Committee (No .003912076)

    【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2007年03期 【DOI】CNKI:SUN:SYJB.0.2007-03-006

     
     
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