王謙; 閆蕾蕾; 王超; 冀宏; 丁萬(wàn)杰; 劉傳斌; 邢志華; 李寶佳
河北大學(xué)生命科學(xué)學(xué)院食藥用真菌研究所; 河北大學(xué)生命科學(xué)學(xué)院食藥用真菌研究所; 河北大學(xué)生命科學(xué)學(xué)院食藥用真菌研究所; 河北省微生物研究所; 河北省微生物研究所; 保定市農(nóng)業(yè)科學(xué)研究所; 保定市農(nóng)業(yè)科學(xué)研究所; 保定市農(nóng)業(yè)科學(xué)研究所 保定071002 ; 保定071002 ; 保定071002 ; 保定071051 ; 保定071051 ; 保定071000 ; 保定071000 ; 保定071000

【中文摘要】 研究表明 ,液體培養(yǎng)條件下 ,茯苓經(jīng) 0～ 4 8h的適應(yīng)期 ,4 8～ 14 4h的增殖期后進(jìn)入穩(wěn)定期 ,發(fā)酵液 pH驟降。經(jīng)檢測(cè) ,發(fā)酵液中含有包括十六烷酸和十八烷酸在內(nèi)的有機(jī)酸 ;發(fā)酵罐培養(yǎng) 12 0h ,生物量≥ 30g/10 0mL(鮮重 )。茯苓營(yíng)養(yǎng)濃縮液多糖含量≥ 5 .0mg/mL ,氨基酸含量≥4 80mg/10 0mL。動(dòng)物實(shí)驗(yàn)證明 ,茯苓營(yíng)養(yǎng)濃縮液具有顯著的抗疲勞和提高機(jī)體免疫力作用 ,其重金屬和微生物指標(biāo)均符合國(guó)家標(biāo)準(zhǔn)。

【英文摘要】 Studies on the submerged culture of Poria cocos showed that followed by 0～48h adaptation period and 48～144h multiplication period, there was the stable growth period with a sudden reduction of pH in the fermentation liquid. The experimental results showed that there were organic acids such as hexadecanoic acid and octadecanoic acid in the fermentation liquid, and that the biomass content was ≥30g/100mL(fresh weight) after 120h fermentation. While the polysaccharide content was ≥5.0mg/100mL and the amino acid content was ≥480mg/100mL in the extracted concentrated nutrient solution of Poria cocos. And the results of the animal tests showed that the effect of concentrated nutrient solution of Poria cocos on the increment of organism immunity and fatigue resistance ability was extremely significant, and the contents of heavy metal and micro organism index accorded with the national standard.

【中文關(guān)鍵詞】 茯苓; 發(fā)酵; 多糖; 免疫; 營(yíng)養(yǎng)濃縮液
【英文關(guān)鍵詞】 Poria cocos; Fermentation; Polysaccharide; Immunity; Concentrated nutrient solution
【基金】河北省科委攻關(guān)項(xiàng)目“茯苓的深層液體培養(yǎng)應(yīng)用研究”(982 4 5 5 0 8D)之部分工作

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2003年02期 【DOI】CNKI:SUN:SYJB.0.2003-02-003