彭衛(wèi)紅; 肖在勤; 甘炳成
四川省農(nóng)業(yè)科學院土壤肥料研究所; 四川省農(nóng)業(yè)科學院土壤肥料研究所; 四川省農(nóng)業(yè)科學院土壤肥料研究所 成都610066 ; 成都610066 ; 成都610066

【中文摘要】 從金針 12 (黃色 )次級菌絲提取細胞核 ,分別與白色金針菇白雪和白色糙皮側耳原生質體在高鈣、高 pH條件下融合。對再生菌絲的研究結果表明 ,無鎖狀聯(lián)合的單核菌絲不形成子實體 ,但雙核化后形成的具有鎖狀聯(lián)合的雙核菌絲則具有形成子實體的能力。試驗結果顯示 ,金針12與白雪組合后代均為黃色金針菇 ;而金針 12與白色糙皮側耳組合后代中 ,大多數(shù)菌株形成的子實體形態(tài)與白色糙皮側耳類似 ,僅 1%～ 2 %菌株形成的子實體形態(tài)與白色金針菇類似

【英文摘要】 Nuclei extracted from secondary mycelia of Flammulina velutipes Jinzhen 12 were fused respectively with the protoplasts of white F.velutipes Baixue and white P.ostreatus under high calcium concentration and high pH conditions.The research result of regenerative mycelia showed that no fruitbody was developed from the monokaroytic mycelia without clamp connections, and that the fruitbody could be developed from the dikaryotic mycelia with clamp connections dikaryolized from monokaryotic mycelia without clamp connection.The experiment result also demonstrated that the fruitbody color of the regenerative strains derived from Jinzhen 12-Baixue combination was all yellow, while the morphology of the fruitbodies produced by most of the regenerative strains derived from Jinzhen 12-P.ostreatus combination was similar to that of the white P.ostreatus, only 1%～2% regenerative strains of this combination produced fruitbodies with a morphology similar to that of the white F. velutipes.

【中文關鍵詞】 金針菇; 轉核; 原生質體核融合; 雙核化
【英文關鍵詞】 Flammulina velutipes; Nucleus transformation; Protoplast nucleus fusion; Dicaryotization
【基金】農(nóng)業(yè)部重點資助項目 (99農(nóng) - 0 6 )

【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2001年03期 【DOI】CNKI:SUN:SYJB.0.2001-03-000