張普照; 舒任庚; 謝小梅
江西中醫(yī)學院現(xiàn)代中藥制劑教育部重點實驗室; 江西中醫(yī)學院現(xiàn)代中藥制劑教育部重點實驗室; 江西中醫(yī)學院菌物藥研究中心 江西南昌330004

【中文摘要】 目的:在前期藥理實驗基礎上,分析雙向性固體發(fā)酵后雷公藤菌質的化學成分。方法:采用高效液相色譜法和薄層色譜法,研究雷公藤藥材和不同發(fā)酵時間藥性菌質的化學成分動態(tài)變化規(guī)律。結果:藥性菌質的雷公藤甲素含量低于原藥材低;分別在218nm和250nm波長下,高溫滅菌藥材和發(fā)酵30d的藥性菌質的液相圖譜有3處明顯的變化;乙酸乙酯提取物的薄層層析發(fā)現(xiàn)有一藍色斑點為發(fā)酵后新增成分,而甲醇提取物薄層層析發(fā)現(xiàn)有2個斑點隨發(fā)酵時間的延長含量降低。結論:雷公藤經藥用真菌發(fā)酵后化學成分發(fā)生變化,其中一些化學成分的變化規(guī)律與藥理實驗結果存在相關性,可作為發(fā)酵終點判斷標準。

【英文摘要】 Objective:On the base of previous pharmacological and toxicological study,chemical compositions in Ttripterygium wilfordii HooK.F after fermentation was compared in this paper.Methods:The content of triptolide in each medicinal material was determined with HPLC and chemical constituents was analyzed with HPLC and TLC.Results:Triptolide content decreased after raw plant herbs was fermented with medicinal fungi,there were three characteristic absorption peaks at wavelength of 218nm and 250nm,one blue spot in TLC chromatograms of ethyl acetate extract and two spots gradually disappeared with the fermentation extent.Conclusion:The of chemical constituents in Tripterygium wilfordii HooK.F happened when medicinal material was fermented,there was relativity of chemical compositions and pharmacological toxicology,consequently,it was regarded as one standard of fermentation termination.

【中文關鍵詞】 雷公藤; 解毒持效; 雙相固體發(fā)酵; 藥用真菌
【英文關鍵詞】 Tripterygium wilfordii HooK.F; The deoxication and retentive-acting; Bi-directional solid fermentation; Medicinal fungal
【基金】國際科技合作項目(2006DFA33060)

【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2008年01期 【DOI】CNKI:SUN:ZSYJ.0.2008-01-017