標題 從赤芝提取的氨基葡聚糖對大鼠實驗性潰瘍的保護作用

英文標題 Protective effects of glycosaminoglycan isolated from Ganoderma lucidum on experimental ulcers in rats

作者 高益槐 新西蘭梅西大學食品、營養和人類健康學院,奧克蘭 候建明 中國海軍青島療養院,山東,青島,266071 藍進 中國海洋大學營養藥物研究所,山東,青島,266003 唐文波 新西蘭天然藥物研究所,奧克蘭 高鶴 新西蘭天然藥物研究所,奧克蘭

作者簡介 高玉槐(1949-),男,教授.研究方向:真菌,天然藥物.

刊名 福建農林大學學報（自然科學版）

英文刊名 JOURNAL OF FUJIAN AGRICULTURE AND FORESTRY UNIVERSITY(NATURAL SCIENCE EDITION)

發表年份 2003

發表卷號 32

發表刊期 2

發表頁碼 245-248

關鍵詞 赤芝 提取物 氨基葡聚糖 大鼠 實驗性潰瘍 保護作用

英文關鍵詞 Ganoderma lucidum P glycosaminoglycan P isolate P rat P experimental ulcer P protective effect

分類號 S853.2,S853.7

文摘 為觀察從赤芝提取的氨基葡聚糖對實驗性潰瘍大鼠的保護作用,采取多步驟從靈芝子實體提取氨基葡聚糖并用色譜法對化學分解產物進行鑒定.大鼠潰瘍造型用應激法(冷水中游泳30 min)、醋酸法、消炎痛法和幽門結扎法誘導產生.氨基葡聚糖以0.5 g@kg-1和1.0 g@kg-1劑量溶于滅菌水中備用,氨基葡聚糖溶液在應用誘導劑后立即用胃管灌胃法給藥,在冷水游泳和幽門結扎前30 min,同法給藥.對照組接受相同量的賦形劑,雷尼替丁以100 mg@kg-1劑量作陽性對照.結果表明:氨基葡聚糖與雷尼替丁對照同樣可以降低胃中氫離子濃度(P＜0.001),其預防潰瘍發生效應較醋酸法和消炎痛法更為顯著;取自靈芝的氨基葡聚糖,是一種具有抗潰瘍的活性多糖,其作用機制有待進一步探討.

英文文摘 To investigate the protective effects of glycosaminoglycans (GAG) from Ganoderma lucidum on experimental ulcersin rats. GAG was isolated from the fruiting body of G. lucidum using a multi-step procedure and the resulting extract was i-dentified by chromatographic methods and chemical analyses of hydrolysates. Rat experimental ulcers were induced by stress(swimming in cold water for 30 min), administration of acetic acid or indomethacin, and ligation of gastric pylorus. GAG (0.5and 1.0 g @ kg-1) was dissolved in sterile water and administered by force-feeding (gastrogarage) immediately before ulcer-in-ducer administration or 30 min before the start of swimming in cold water and ligation of gastric pylorus. Control animals re-ceived the same amount of vehicle. Ranitidine (100 mg @ kg-1, p. o. ) was used positive control. The results indicated thatGAG had similar effects on the reduction of the ulcer formation and free gastric H+ levels (P＜0.001) compared with raniti-dine-treated group. The preventive effects appeared to be more marked in the acetic acid- and indomethacin-treated groups.These results indicate that GAG isolated from G. lucidum is an active polysaccharide component with ulcer-healing effects.Further studies are required to explore the mechanism of the action of GAG.

基金項目 新西蘭自然科學基金.

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