洛巴口蘑濾液中多糖蛋白復合物的抗腫瘤機制
ANTITUMOR MECHANISM OF POLYSACCHARIDE-PROTEIN COMPLEX FROM THE CULTURE FILTRATE OF TRICHOLOMA LOBAYENSE
劉方 黃容春 邢來君 趙力 張樹庭
摘 要：從洛巴口蘑培養濾液中分離出一種具有抗腫瘤性的多糖蛋白復合物(PSPC).PSPC在細胞水平有能力恢復和增強帶瘤鼠腹腔浸出細胞的吞噬功能和T細胞的促分裂活性.在分子水平,PSPC能恢復帶瘤鼠脾細胞和腹腔浸出細胞內IFN-γ基因的表達水平.另外,PSPC能夠下調帶瘤鼠脾細胞內"抗細胞因子”TGF-β的表達水平.因此,PSPC的抗腫瘤機制可歸咎于寄主介導的免疫調節作用.
關鍵詞：多糖,蘑菇,細胞因子,免疫調節
分類號：Q939.93 文獻標識碼：A
文章編號：1007-3515-62000)03-0396-0400
作者單位：劉方（天津南開大學微生物系 天津 300071）
黃容春（香港中文大學生物系 香港）
邢來君（天津南開大學微生物系 天津 300071）
趙力（天津醫院 天津 300211）
張樹庭（香港中文大學生物系 香港）
參考文獻：
［1］Abbas A K,Lichtman A H,Pober J S,1991.Cellular and Molecular Immunology.Philadelphia:W.B.Sannders
［2］Berg D J,Lynch R G,1991.Immune dysfunction in mice with plasmacytomas,I.Evidence that transforming growth factor-βcontributes to the altered expression of activation receptors on host B lymphocytes.The Journal of Immunology,146:2865～2872.
［3］Callard R E,1990.Cytokines and B Lymphocytes.London:Academic Press
［4］Chang R,1996.Functional properties of edible mushroom.Nutrition Reviews,54:91～93.
［5］Chang S T,Mao.X L,1995. Hong Kong Mushrooms.Hong Kong:The Chinese University of Hong Kong Press,79～80.
［6］Kehrl J H, Roberts A B,Wakefield L M,et al.,1986.Transforming growth factor beta is an important immunomodulatory protein for human B lymphocytes.The Journal of Immunology,137:3855～3860.
［7］Leung M Y,Fung P K,Choy Y M,1997.The isolation and characterization of an immunomodulatory and antitumor polysaccharide preparation from Flarmmulina velutipees.Immunopharmacology,35:255～263.
［8］Liu F,Ooi V E C,Chang S T,1995.Antitumor components of the culture filtrates from Tricholoma sp.World Journal of Microbiology and Biotechnology,11:486～490.
［9］Liu W K,Mak N K,Wong C C,1989.Immunomodulatory effect of methimazole on inbred mice.Immunobiology,18:23～32.
［10］iu W K,Ng T B,Sze S F,et al.,1993.Activation of peritoneal macrophages by polysaccharopeptide from the mushroom,Coriolus versicolor.Immunopharmacology,26:139～146.
［11］Mizuno T,Saito H,Nishitoba T,et al.,1995.Antitumor-active substances from mushrooms.Food Review International,11:23～61.
［12］Shibata Y,Tamura K,Ishida N,1983.In vivo analysis of the suppressive effects of immunosuppressive acidic protein,a type of alfa-1 acid glycoprotein,in connection with its high level in tumor-bearing mice.Cancer Research,43:2889～2896.
