周洪英; 邊銀丙
湖北省農(nóng)業(yè)科學(xué)院經(jīng)濟(jì)作物研究所; 華中農(nóng)業(yè)大學(xué)應(yīng)用真菌研究所 武漢430070; 武漢430070

【中文摘要】 以蛹蟲草(Cordyceps militaris)4號(hào)和8號(hào)菌株作為親本,采用滅活原生質(zhì)體融合技術(shù)獲得228個(gè)再生菌株。酯酶同工酶與ISSR-PCR方法相結(jié)合鑒定融合子,以蟲草素含量和子實(shí)體性狀為篩選指標(biāo),經(jīng)過初步的遺傳穩(wěn)定性測(cè)試,得到蟲草素含量顯著提高的5個(gè)穩(wěn)定融合子——CM59、CM61、CM69、CM136和CM186 ,其蟲草素含量分別為8號(hào)菌株的1 .17 1 .73倍。實(shí)驗(yàn)證明融合子菌絲中蟲草素含量普遍提高。篩選得到的5個(gè)融合子經(jīng)進(jìn)一步遺傳穩(wěn)定性驗(yàn)證后可用于以獲得蟲草素為目的的菌絲體發(fā)酵培養(yǎng)。

【英文摘要】 Protoplast fusion was carried out between protoplasts prepared from two parental strains of Cordyceps militaris (strains 4 and 8) after the protoplasts from both parental types had first been inactivated either by exposure to 60 ℃ or to UV irradiation. A total of 228 putative fusion products were obtained following protoplast regeneration and confirmed as true protoplast-fusion products by esterase isozyme analysis and ISSR-PCR methodology. Five regenerated fusion products, designated CM59, CM61, CM69, CM136 and CM186, were selected for increased and stable cordycepin production capacity. Increased cordycepin production ranged from between 1.17～1.73-fold higher compared with the higher cordycepin-yielding parental strain 8. While most of the fusion products exhibited increases in cordycepin content, the five selected isolates appear to have a high potential for producing cordycepin in fermentation systems subject to further testing of genetic stability.

【中文關(guān)鍵詞】 滅活原生質(zhì)體; 原生質(zhì)體融合; 鑒定; 蟲草素; 遺傳穩(wěn)定性
【英文關(guān)鍵詞】 Inactivated protoplast; Protoplast fusion; Cordycepin; Genetic stability
【基金】華中農(nóng)業(yè)大學(xué)各類人才科研啟動(dòng)項(xiàng)目(No52204-05021)的部分研究?jī)?nèi)容

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2007年02期 【DOI】CNKI:SUN:SYJB.0.2007-02-016