王曉娥; 姚方杰
吉林農(nóng)業(yè)大學(xué)園藝學(xué)院; 吉林農(nóng)業(yè)大學(xué)園藝學(xué)院 吉林長(zhǎng)春130118; 吉林工程技術(shù)師范學(xué)院生物與食品工程系; 吉林長(zhǎng)春130052; 吉林長(zhǎng)春130118
【中文摘要】 以我國(guó)云南和四川產(chǎn)的5個(gè)塊菌菌株為試驗(yàn)材料,提取塊菌DNA并對(duì)rDNA的ITS序列分析試驗(yàn)條件的初步研究,結(jié)果表明:運(yùn)用常規(guī)CTAB法提取塊菌子實(shí)體DNA,獲得的DNA產(chǎn)量高,可以滿(mǎn)足本試驗(yàn)的要求;明確了rDNA ITS區(qū)域的適宜PCR擴(kuò)增和克隆條件;獲得4個(gè)長(zhǎng)度為650bp、1個(gè)長(zhǎng)度為800bp的PCR產(chǎn)物;將PCR產(chǎn)物連接到PMD18-T載體上,轉(zhuǎn)化E.coliJM109,獲得了陽(yáng)性克隆,供進(jìn)一步序列分析用。
【英文摘要】 5 truffle samples clustered from Sichuan and Yunnan were selected to extract DNA and the internal transcribed spacer(ITS) regions of nuclear ribosomal DNA was amplified.The results showed: The optimal PCR and clone conditions were assured;The length of 4 samples was 650bp and1 sample was 800bp;The PCR product was linked to PMD18-T vector and transformed into E.coliJM109.We chose the positive clone which would use in the further sequence analysis.
【中文關(guān)鍵詞】 塊菌; rDNA; PCR; 克隆
【英文關(guān)鍵詞】 Truffle; rDNA; PCR; Clone
【基金】農(nóng)業(yè)部“98”資助項(xiàng)目(項(xiàng)目編號(hào)2001-21)
【文獻(xiàn)出處】 中國(guó)食用菌,Edible Fungi of China,編輯部郵箱,2006年05期 【DOI】CNKI:SUN:ZSYJ.0.2006-05-015