施慶利; 羅信昌
華中農(nóng)業(yè)大學(xué)應(yīng)用真菌研究所

【中文摘要】 本研究以ＲＡＰＤ（ＲａｎｄｏｍｌｙＡｍｐｌｉｆｉｅｄＰｏｌｙ－ｍｏｒｐｈｉｃＤＮＡ）技術(shù)為主，比較分析食用菌木耳屬（Ａｕｒｉｃｕｌａｒｉａ）種內(nèi)雜交種．種間原生質(zhì)體融合子與其親本菌株的ＰＣＲ（ＰｏｌｙｍｅｒｓｅＣｈａｉｈＲｅａｃｔｉｏｎ）擴(kuò)增的ＤＮＡ片段之間差異，并輔以其它手段，如核相及核數(shù)目觀察，同功酶鑒別，以期建立完整有效的檢測(cè)技術(shù)系列，確證其雜交種融合子的真實(shí)性及可靠性。在ＲＡＰＤ分析中，用于測(cè)試的１０種隨機(jī)引物僅有４種引物與供試菌株的ＤＮＡ模板具有較好的結(jié)合能力，其中１種引物可用于檢測(cè)雜交種及其親本，另外３種引物可用于檢測(cè)融合子反親本。檢測(cè)結(jié)果顯示：３個(gè)供試雜交種中有２個(gè)為真雜合子，１個(gè)為假雜合子；２個(gè)供試融合子均為非穩(wěn)定雜合子。與常規(guī)方法鑒定結(jié)果比較基本相符。因此，ＲＡＰＤ技術(shù)提供了一種快速、簡(jiǎn)便、準(zhǔn)確的雜交種及融合子的鑒別方法。

【英文摘要】 RAPD(Randomy Amplified Polymorphic DNA)was used to analysethe differences of DNA fragments between intraspecies hybrid,interspecies protoplastfusants and their respective parents in genus Auricularia,With the help of other methodssuch as the nuclear number in the hyphal cell, the comparison of the isozyme graphs, the hybrids and the protoplast fusants were identified.Four of ten arbitrary primets had good compatibility with DNA template of test strainsin RAPD assay. One of four was used to dentify by brids and their parents, three of fourwere used to identify fusants and their parents. The results showed that two of threehybrids were real heterozygotes,the two fusants were not stable.Therefore,comparing with the other ways,RAPD provided a rapid, simple, accurateidentified method of hybrids and fusants.

【中文關(guān)鍵詞】 本耳屬; RAPD; PCR; 雜合子; 融合子
【英文關(guān)鍵詞】 Auricularia; RAPD; PCR; Hybrid; Fusant
【基金】農(nóng)業(yè)部“八五”生物技術(shù)攻關(guān)項(xiàng)目

【文獻(xiàn)出處】 中國(guó)食用菌,EDIBLE FUNGI OF CHINA,編輯部郵箱,1994年06期 【DOI】CNKI:SUN:ZSYJ.0.1994-06-000