豬苓、伴生菌及蜜環菌共培養的形態學研究

MORPHOLOGICAL STUDIES ON DUAL AND TRIPLE CULTURE OF GRIFOLA UMBELLATA, AN UNNAMED COMPANION FUNGUS AND ARMILLARIELLA MELLEA

邢曉科　郭順星　

摘　要：本文對豬苓、伴生菌及蜜環菌兩兩共培養及三者共培養進行了宏觀形態觀察及細胞學水平上的研究.結果表明,豬苓與伴生菌共培養時,在二者之間形成一致密拮抗線,豬苓菌落表面菌絲分化產生大量菌絲束;豬苓與蜜環菌共培養時,豬苓能阻止蜜環菌菌索對其自身的進一步侵襲,互作區中的雙方菌絲及菌索均停止生長;蜜環菌與伴生菌共培養時,蜜環菌能穿透整個伴生菌菌落,在伴生菌菌落下方產生大量分枝;三者共培養后,豬苓對蜜環菌的防御能力有所下降,伴生菌對蜜環菌的耐受力有所提高,蜜環菌產生的新分枝均向伴生菌一側生長,豬苓與伴生菌之間并不形成致密拮抗線,只可見雙方菌絲的白色交融區.豬苓與伴生菌均能在蜜環菌菌索皮層上形成侵入位點.

關鍵詞：雙重培養,三重培養,培養性狀

分類號：Q939.5 　文獻標識碼：A

文章編號：1007-3515(2003)04-0653-0660

基金項目：國家自然科學基金資助項目(no.39570012)

作者簡介：郭順星,通訊作者

作者單位：邢曉科（中國協和醫科大學,中國醫學科學院,藥用植物研究所,北京,100094）　

　　　　　郭順星（中國協和醫科大學,中國醫學科學院,藥用植物研究所,北京,100094）　

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