Artificial Cultivation of Medicinal Mushroom, Phellinus linteus Using Mulberry Log
Abstract：For comparison of the mycelial colonization of Phellinus linteus on log, the sterilized short log inoculation, drilling inoculation and log-end sandwich inoculation were conducted. The mycelial colonization of P. linteus on logs was good in the sterilized short log inoculation, but traditional method such as drilling inoculation and log-end sandwich inoculation were poor. The initial mycelial growth and the full mycelial colonization of P. linteus were best on 20 cm of short log under the condition of 42% of moisture content in log. Also the initial mycelial growth of P. linteus was accelerated over 12 hours of sterilization. Burying method after 5～6 months incubation of log was best in the basidiocarp formation of P. linteus. The fruiting body formation of P. linteus was quite good in the cultivation house controlled at the temperature range of 31～35?B℃ and over 96% of relative humidity.
Keywords：Phellinus linteus ; the sterilized short log inoculation; drilling inoculation ; log-end sandwich inoculation; mycelial colonization
利用桑木人工栽培藥用真菌靈芝(Phellinus linteus)的研究
Hong In Pyo Sung Gyoo Byung Nam Hack Woo
摘 要：比較了利用無菌短木接種法、鉆孔接種法和端木夾心接種法等接種方法接種桑樹段木后Phellinus linteus的菌絲生長和定殖狀況.研究表明利用滅菌的短木接種P.linteus,生長和定殖良好,傳統(tǒng)的鉆孔接種法和端木夾心接種法菌絲生長定殖不好.P.linteus在20 cm長的桑木上生長和定殖所需的最適的含水量為42%,接種后12 h,P.linteus立即進入快速生長期.接種后的桑木埋入土壤中需要5～6個月產(chǎn)生擔子果,在培養(yǎng)室內(nèi)溫度為31～35?B℃,相對濕度大于96%時有利于子實體的形成.
關鍵詞：靈芝(Phellinus linteus); 無菌短木接種法; 鉆孔接種法; 端木夾心接種法; 菌絲定殖
CLC Number：S567.3+1
Foundation Item：Supported by NIAST of Suwon, Korea
Author Resume：Hong In Pyo,male,researcher.Address:Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology, R.D.A., Suwon 441-707, Korea
Author Unit：Hong In Pyo（Department of Sericulture and Entomology,National Institute of Agricultural Science and Technology,R.D.A.,Suwon 441-707,Korea）
Sung Gyoo Byung（Department of Sericulture and Entomology,National Institute of Agricultural Science and Technology,R.D.A.,Suwon 441-707,Korea）
Nam Hack Woo（Department of Sericulture and Entomology,National Institute of Agricultural Science and Technology,R.D.A.,Suwon 441-707,Korea）
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華中農(nóng)業(yè)大學學報
JOURNAL OF HUAZHONG AGRICULTURAL UNIVERSITY
2004 Vol.23 No.1
