秦小明; 余娟; 寧恩創; 林華娟
湛江海洋大學食品科技學院; 廣西大學輕工與食品工程學院; 廣西大學輕工與食品工程學院; 湛江海洋大學食品科技學院 湛江524088 ; 南寧530004 ; 南寧530004 ; 湛江524088

【中文摘要】 初步探討了金針菇子實體多糖成分的分離與純化。采用熱水抽提、三氯乙酸法脫蛋白、乙醇沉淀獲得金針菇子實體粗多糖,然后經DEAE Cellulose陰離子交換柱(B2O74-型)進行分離純化。結果表明,金針菇粗多糖是一個以葡聚糖為主、由數個多糖組分構成的混合多糖。

【英文摘要】 <Abstrcat> Primary isolation and purification of polysaccharide compositions of Flammulina velutipes was studied. Flammulina velutipes crude polysaccharide was extracted by hot water, deprived of proteins using trichloroacetic acid method and precipitated by ethanol, then the crude polysaccharide was isolated and purificated into three fractions——FVCP-A, FVCP-B and FVCP-C by DEAE-Cellulose column chromatography (B_2O_74-). The result of monosacharide composition analysis suggested that F. velutipes crude polysaccharides should be the mixed polysaccharides mainly containing glucan and other components.

【中文關鍵詞】 金針菇; 葡聚糖; 分離純化
【英文關鍵詞】 Flammulina velutipes; Glucan; Isolation and purification

【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2005年02期 【DOI】CNKI:SUN:SYJB.0.2005-02-006