姜文俠; 孫武岳; 馬琳; 呂呈剛; 易雙全
天津市工業微生物研究所; 天津市工業微生物研究所; 天津市工業微生物研究所; 天津市工業微生物研究所; 天津市工業微生物研究所 天津 300221 ; 天津 300221 ; 天津 300221 ; 天津 300221 天津輕工業學院畢業實習生 ; 天津 300221 天津輕工業學院畢業實習生

【中文摘要】 試驗研究了冬蟲夏草(Cordyceps sinensis)蝙蝠蛾擬青霉(Paecilomyces hepiali Chen et Dai)菌絲體的發酵條件,其適宜接種量為2.5％～5.0％(v/v),最佳菌齡為3d,適宜的初始pH為5.0～7.5,發酵周期5d,發酵過程中對通氣量的要求較高。以20m3發酵罐培養,菌絲體收率為2.5％,其D-甘露醇含量8.3％,腺苷含量0.24％。

【英文摘要】 The mycelial fermentation conditioas of Paecilomyces hepiali Chen et Dai of Cordyceps sinensis were studied in our last. The mycelia were cultured at 24℃ on a rotary shaker at 120 r/min for 5 days. It was proved that the suitable initial pH of the medium was 5.0 - 7.5, the inoculation ratio was 2.5% -5.0%(v/v) and the optimal spawn age was 3 days, and that more attention should be paid to the aeration during the fermentation process. Fermented in 20m3 fermentor, the yield of mycelia was 2. 5% .among which the content of D- mannitol in the dried mycelium was 8.3% and the content of adenosine was 0. 24% .

【中文關鍵詞】 蝙蝠蛾擬青霉; 菌絲體發酵; 冬蟲夏草
【英文關鍵詞】 Paecilomyces hepiali ; Mycelial fermentation; Cardyceps sinensis

【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,1999年02期 【DOI】CNKI:SUN:SYJB.0.1999-02-008