陳體強; 李開本; 朱培根; 徐潔; 何修金; 陳曦; 陳朝旭
福建省農業科學院原木靈芝發展中心; 福建省農業科學院原木靈芝發展中心; 福建省農業科學院原木靈芝發展中心; 福建省農業科學院原木靈芝發展中心; 福建省農業科學院原木靈芝發展中心; 福建省測試技術研究所; 福建省測試技術研究所 福州 350013 ; 福州 350013 ; 福州 350013 ; 福州 350013 ; 福州 350013 ; 福州 350003 ; 福州 350003

【中文摘要】 原木靈芝孢子與木屑袋栽靈芝孢子可以鑒別如下:1.在掃描電鏡下,前者形態飽滿,后者大多數孢壁內陷;2.X-射線能譜面掃描分析結果表明,兩者Mg、Al、Si、P、S、Cl、K、Ca、Fe、Ni等無機元素相對含量差異顯著,前者Si、Ca含量較高;3.高效液相色譜分析結果表明,前者靈芝酸(Ganoderic acid)A、B等靈芝三萜酸含量較高,而后者未檢出Ganoderic acid C;4.孢子粉經乙醚脫脂后用乙醇回流提取、濃縮成浸膏,前者提取率(固形物含量)達4.2％～4.8％,而后者僅為1.9％～2.1％。

【英文摘要】 Spores of log-cultivated Ganoderma lucidum and spores of sawdust-bag-cultivated G. lucidum could be identified as follows: 1. It was obvious that the majority of the former were plump and most of the latter looked shrivelled under ESM. 2. The relative content of inorganic elements, such as Mg, Al, Si, P, S, Cl, K, Ca, Fe, Ni, was analysed by X-ray EDS. The results showed that there was significant difference between these two kinds of spores, and that the former had higher content of Si and Ca. 3. HPLC analysis results showed that content of ganoderic acid A, B and other triterpenoid acid from the former was higher than that of the latter, but ganoderic acid C was not checked out from the latter. 4. Both kinds of the spores were extracted by alcohol reflux after being degreased by ether, and then they were concentrated. The results showed that extract rate (content of solid state substance) of the former was 4.2% -4.8%, while the latter was only 1.9%- 2.1%.

【中文關鍵詞】 靈芝孢子; 顯微形態; 無機元素; 三萜酸; 孢子粉提取率
【英文關鍵詞】 Ganoderma lucidum spore; Micro-morphology; Inorganic element; Triterpenoid acid; Alcohol extract

【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,1998年01期 【DOI】CNKI:SUN:SYJB.0.1998-01-009