王立波; 孫東植; 鐘秀宏; 金仁君; 陳海月
吉林延邊大學(xué)基礎(chǔ)醫(yī)學(xué)院病理學(xué)與法醫(yī)學(xué)教研部; 吉林延邊大學(xué)基礎(chǔ)醫(yī)學(xué)院病理學(xué)與法醫(yī)學(xué)教研部; 吉林醫(yī)藥學(xué)院病理教研室; 吉林延邊大學(xué)基礎(chǔ)醫(yī)學(xué)院病理學(xué)與法醫(yī)學(xué)教研部; 吉林延邊大學(xué)基礎(chǔ)醫(yī)學(xué)院病理學(xué)與法醫(yī)學(xué)教研部 延吉133000; 延吉133000; 吉林132013; 延吉133000; 延吉133000

【中文摘要】 用MTT法測(cè)定樺褐孔菌抗突變活性成分Inotodiol,對(duì)人肺癌A549細(xì)胞株的增殖抑制作用,20mg.L-180mg.L-1樺褐孔菌的抗突變活性成分Inotodiol對(duì)人肺癌A549細(xì)胞株有抑制增殖作用,且表現(xiàn)出濃度和時(shí)間依賴關(guān)系。在倒置顯微鏡下、HE染色光學(xué)顯微鏡下、AO/EB雙染色熒光顯微鏡下、透射電子顯微鏡下觀察,腫瘤細(xì)胞均有體積變小、有凋亡小體。樺褐孔菌的抗突變活性成分Inotodiol可抑制人肺癌A549細(xì)胞株的增殖和誘導(dǎo)腫瘤細(xì)胞凋亡。

【英文摘要】 A549 human lung cancer cell lines were dealt with anti-mutation active constituents from Inonotus obliquus Inotodiol at different concentrations.Cell proliferation was determined by MTT.Anti-mutation active constituents from Inonotus obliquus in concentrations of 20mg·L-1,40mg·L-1,80mg·L-1 could inhibite the hyperplasis of A549 human lung cancer cells in a doseage and time dependent relation.Cells of tumour were small,and there was apoptosis under the observation of transmission microscope and HE staining light microscope and AO/EB double staining fluoresent microscope and transmission electron microscope.Anti-mutation active constituents from Inonotus obliquus Inotodiol has the effects of anti-proliferation and apoptosis on A549 human lung cancer cells.

【中文關(guān)鍵詞】 樺褐孔菌; 肺腫瘤; 細(xì)胞凋亡; 細(xì)胞增殖
【英文關(guān)鍵詞】 Inonotus obliquus; Lung cancer; Celluar apoptosis; Celluar proliferation

【文獻(xiàn)出處】 中國(guó)食用菌,Edible Fungi of China,編輯部郵箱,2007年04期 【DOI】CNKI:SUN:ZSYJ.0.2007-04-015