李林輝
四川師范學院生物系 四川南充637002

【中文摘要】 本研究以兩頭洞穿的玻璃管替代常規玻璃試管 ,制備兩端營養較豐富而中間段營養貧瘠但富含木質素纖維素的夾心面包式固體培養基 ,利用大多數大型真菌分解利用木質素纖維素能力較強的特點 ,采用在一端接入野外不能嚴格消毒的子實體 ,卻在另一端取出已純化母種的方法 ,過濾掉雜菌 ,富集到目的大型真菌 ,為那些遠離實驗室地區考察短期內不能將野外采集到的大型真菌立即送回實驗室按現行普遍采用的方法立即進行分離而獲得母種的大型真菌工作者提供了一個可資利用的新手段

【英文摘要】 This study provides a technology for the researchers who are working outdoors far from laboratory,who want to stay outdoors for longer,having no time to separate strains immediately from the macrofungi they dug by the popular method nowadays.The progress expresses as follows:preparing a glassy tube instead of popular one which is through,mixing similar-hamburger solid media which provides a abundant nutrition in both end where many animalcule may grow and spare one in the middle where only those animalcule which has the ability analyzing lignin and cellulose surely including macro fungi can grow,sterilization strictly,inoculating macrofungi which can not be sterilized strictly in one end of the media,culturing,a time latter,transferring pure hypha in another end of the media to PDA media or other media.

【中文關鍵詞】 大型真菌; 菌種; 技術; 分離; 野外
【英文關鍵詞】 Macrofungi; Strain; Technology; Separate; Outdoors

【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2002年05期 【DOI】CNKI:SUN:ZSYJ.0.2002-05-006